反相-高效液相色谱法测定黄龙止咳颗粒中盐酸麻黄碱和盐酸伪麻黄碱的含量

Determination of Ephedrine Hydrochloride and Pseudoephedrine Hydrochloride in Huanglongzhike Granule by RP-HPLC

目的：建立反相-高效液相色谱法（RP-HPLC）测定黄龙止咳颗粒中盐酸麻黄碱和盐酸伪麻黄碱含量的方法.方法：色谱柱：AgilentZORBAXSB-C18色谱柱（4.6mm×250mm,5μm）;流动相：乙腈-磷酸二氢钾溶液（4∶96）（取磷酸二氢钾6.8g,加水1000ml溶解后,加三乙胺1ml,用磷酸调节pH值至3.0）;检测波长：210nm;柱温：35℃.结果：盐酸麻黄碱、盐酸伪麻黄碱进样量分别在0.0040～0.0480μg（r=0.9996）、0.0237～0.2844μg（r=0.9999）范围内线性关系良好;平均回收率分别为96.2％、97.5％,RSD分别为1.97％、1.81％.结论：本方法简便、准确,适用于黄龙止咳颗粒中盐酸麻黄碱和盐酸伪麻黄碱的含量测定.

OBJECTIVE：To establish a RP-HPLC method for determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Huanglongzhike granule. METHODS： The samples were separated on Agilent ZORBAX SB-C is column （4.6 mmx 250 mm, 5 μm） with mobile phase composed of CH3 CN-KH2 PO4 solution （ KH2 PO4 6. 8 g dissolved in 1 000 ml of water followed by addition of 1 ml triethylamine, with pH adjusted to 3.0 by phosphoric acid） （4： 96）. Ephedrine hydrochloride and Pseudoephedrine hydroehloride were detected at 210 nm. The column temperature was set at 35 ~C. RESULTS ： The linear range was 0. 004 0-0. 048 0 μg （ r = 0. 999 6 ） for ephedrine hydrochloride and 0. 023 7-0. 284 4 μg（ r = 0. 999 9） for pseudoephedrine hydrochloride. The average recoveries of ephedrine hydrochloride and pseudoephedrine hydrochloride were 96. 2% and 97.5%, respectively with RSD of1.97% and 1.81%, respectively. CONCLUSION： The method is simple and accurate and it can be used for determining the contents of ephedrine hydrochloride and pseudoephedrine hydrochloride in Huanglongzhike granule.