核转录因子κb在宫腔粘连大鼠模型中的表达

The expression of nuclear transcription factor κb in rat model of intrauterine adhesions

目的建立大鼠宫腔粘连模型并检测大鼠血液和组织中核转录因子κb(nuclear transcription factor kappa B,NF-κb)的含量,探讨NF-κb与宫腔粘连的关系。方法用苯酚胶浆法建立宫腔粘连动物模型,HE染色观察病理学变化,免疫组化染色法检测大鼠子宫内膜NF-κb亚基p65蛋白质的表达,Real-time PCR方法检测NF-κb p65 mRNA的表达,ELISA双抗夹心法检测血液中NF-κb p65蛋白质的变化。结果 NF-κb亚基p65蛋白质主要定位于细胞核,在宫腔粘连组的表达量明显高于正常组和假手术组(P<0.05),正常组和假手术组间无差异(P>0.05);NF-κb p65 mRNA在宫腔粘连组的表达量明显高于正常组和假手术组(P<0.05),正常组和假手术组无差异(P>0.05);血液中NF-κb亚基p65蛋白质的表达量在宫腔粘连组高于正常组和假手术组(P<0.05),正常组和假手术组间无差异(P>0.05)。结论宫腔粘连的大鼠,NF-κb亚基p65的表达量明显增高,提示NF-κb介导的信号通路在宫腔粘连的发病机制中起重要作用。

Objective To build rat model of intrauterine adhesions,to detect the expression of NF - Kb p65 in uterine tissue and plasma, and to explore the relationship between NF- Kb and intrauterine adhesions. Methods Rat intrauterine adhesions model was set up by phenol mucilage. Histologic changes of uterine were monitored by hematoxylin eosin, NF- Kb p65 protein expression of semi -quantitative and location in the rat endometrium were assessed by immunohistochemical staining. The changes of NF - Kb p65 mRNA expression were assayed by real - time PCR, NF - Kb p65 protein expression in the blood was detected by ELISA. Results The NF - Kb p65 protein was located in the nucleus, the expression of NF - Kb p65 protein of the intrauterine adhesions group was significantly higher than that of normal group and control group （ P 〈 O. 05 ） , no significant difference was found between normal group and control group （ P 〉 O. 05 ） ; The p65 mRNA of NF - Kb of the intrauterine adhesions group expressed significantly higher than that of normal group and control group （ P 〈 0.05 ） , and no significant difference was found between normal group and control group （ P 〉 0. 05 ） ; The expression of NF - Kb in blood of intrauterine adhesions group were significantly higher than that of normal group and control group （ P 〈 0.05 ） , and no significant differenceswas found between the two groups （ P 〉 0. 05 ）. Conclusion adhesions model increased obviously which indicated NF - Kb mediated inflammatory factors. The expression of NF - Kb in the rat intrauterine plays an important role in intrauterine adhesion as