中华圆田螺多糖对小鼠酒精性肝损伤的保护作用

Protective effect of Cipangopaludina chinensis polysaccharide on alcoholic hepatic injury in mice

目的探讨中华圆田螺多糖对小鼠酒精性肝损伤的保护作用。方法将昆明种小鼠随机分成6组,分别为健康对照组、模型对照组、阳性对照组、中华圆田螺多糖低剂量组、中剂量组和高剂量组。模型组小鼠用56℃红星二锅头12ml/(d·kg)体重灌胃,连续10d;药物组在给予同等量酒精灌胃的同时分别用低剂量、中剂量和高剂量中华圆田螺多糖灌胃,连续10d;阳性对照组按80mg/(d·kg)体重灌胃益肝灵;健康对照组每天用等量生理盐水灌胃。末次灌胃后摘眼球取血,分离血清,测定血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性;处死小鼠,取肝脏,制成肝匀浆,检测超氧化物歧化酶(SOD)活性剂及丙二醛(MDA)和还原型谷胱甘肽(GSH)的含量。同时光镜下观察肝组织病理形态学的改变。结果中华圆田螺多糖中、高剂量组小鼠血清ALT、AST分别为(46.22±10.61、43.06±11.18)U/L和(135.64±24.53、119.17±23.17)U/L,与模型组(66.71±15.46)U/L和(196.22±42.13)U/L比较差异有统计学意义(P<0.05或P<0.01);中华圆田螺多糖低、中、高剂量组小鼠肝脏SOD和MDA分别为(161.36±15.67、170.69±14.73、175.12±16.77)U/mg prot和(1.37±0.39、1.18±0.26、1.10±0.28)nmol/mg prot,与模型组(140.86±13.36)U/mg prot和(1.84±0.57)nmol/mg prot比较差异有统计学意义(P<0.05或P<0.01);中华圆田螺多糖中、高剂量组GSH为(237.44±43.37、248.96±33.10)nmol/mg prot,与模型组(202.62±39.88)nmol/mg prot比较差异有统计学意义(P<0.05)。肝脏病理切片显示中华圆田螺多糖各剂量组对酒精性肝损伤小鼠肝细胞变性、坏死、炎性细胞浸润程度有改善作用。结论中华圆田螺多糖能降低血清ALT、AST活性和肝脏MDA含量,增加血清SOD活性,提高肝脏GSH含量,对酒精性肝损伤具有一定保护作用。

Objective To investigate the protective effect of Cipangopaludina chinensis polysaccharide on alcoholic he- patic injury in mice. Methods Kunming mice were randomly divided into 6 groups： a normal control group, a model group, a positive control group, a group given a low dose of C. chinensiss polysaccharide, a group given a medium dose, and a group given a high dose. Mice in all groups except the normal control group were perfused with the 56 C Red Star white wine （12 ml/kg） for 10 d to cause alcoholic hepatic injury. The three groups given C. chinensis polysaccharide were given different doses of C. chinensisn polysaecharide once a day for 10 d. The positive control group was perfused with the traditional Chinese medicine Yiganling （80 mg/kg）. The normal control group was given an equal volume of saline for the same duration. After the latest perfusion, blood was drawn from the eyeball, and levels of serum ALT and AST were de- termined. A liver homogenate was prepared to detect MDA and GSH content and SOD activity. Pathological changes in liver tissue were observed. Results Groups given the medium dose and high dose of C. chinensis polysaccharide had lower serum levels of ALT and AST （ALT： 46. 22±10.61 and 43.06±11. 18; AST： 135.64±24.53 and 119. 17± 23.17） compared to the model group （ALT： 66.71±15.46; AST： 196.22±42.13, 13〈0.05 orP〈0.01）. Groups giv- en the low, medium, and high dose of C. chinensis polysaccharide had increased SOD activity （161.36±15.67, 170.69± 14.73, and 175.12±16.77） compared to the model group （140. 86±13.36, P〈0. 05）. Groups given the medium and high dose of C. chinehsis had increased GSH content （237.44±43.37 and 248.96±33.10） compared to the model group （202.62±39.88, P〈0.05）. Groups given the low, medium, and high dose of C. chinensis polysaccharide had decreased MDA content （1.37＋0.39, 1. 18±0.26, and 1.10±0.28） compared to the model group （1.84±0.57, P〈0. 05 or P〈 0.01）. C. chinensis polysaccharide significantly alleviated damage to liver tissue. Conclusion C. chinensis pysaccharide has action to protect the liver from alcoholic injury.