摘要
目的:通过观察消异方对子宫内膜异位症大鼠异位内膜细胞凋亡倾向的影响,初步探讨消异方治疗子宫内膜异位症的机制。方法:采用自体手术移植法建立大鼠子宫内膜异位症模型,随机分为模型组、米非司酮组、散结镇痛胶囊组、消异方低、中、高剂量组。造模成功各组给药4 W,异位内膜细胞凋亡TUNEL检测、免疫组织化学检测Caspase-3及survivin。结果:消异方各组异位内膜细胞凋亡率均高于模型组,有显著性差异;异位内膜的Caspase-3表达比模型组显著性提高,survivin表达比模型组显著性降低。结论:消异方明显抑制异位内膜生长的作用可能是通过诱导异位内膜细胞凋亡的方式来实现的。
Objective ： By observing the effect of Xiaoyi Formula on the apoptosis of ectopic endometrial cells of endo- metriosis rats to investigate the mechanism of Xiaoyi Formula in the treatment of endometriosis. Methods ： After the success of modeling, the rats were randomly divided into 6 groups： model group, Sanjie Zhentong Capsule group, mifepristone group,low dose of Xiaoyi Formula group, middle dose of Xiaoyi Formula group, high dose of Xiaoyi Formula group. Four weeks after medication, the apoptosis rate of ectopic endometrial cells was detected by TUNEL and Caspase -3 and sur- vivin were tested by immunohistochemistry method. Result ：The apoptosis rate of ectopic endometrial ceils and Caspase -3 in model group was lower than that of each Xiaoyi Formula groups. The survivin in model group was higher than that of each Xiaoyi Formula groups. Conclusion ： Xiaoyi Formula can significantly restrain the growth of ectopic endometrium by inducing apoptosis of ectopic endometrial cells.
出处
《中华中医药学刊》
CAS
2014年第4期769-771,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家中医药管理局重点学科项目(200910)
陕西省教育厅科学基金项目(08JK268)
陕西省科技厅自然科学研究基础项目(青年项目)(2010JQ4008)
