对NaCl敏感度不同的二倍体马铃薯在NaHCO_3胁迫下的表现

Performance of Diploid Potatoes with Various Levels of NaCl Tolerance under Stress of NaHCO_3

马铃薯(Solanum tuberosum L.)是对盐中度敏感的作物。二倍体马铃薯存在着丰富的遗传变异,是耐盐碱育种的宝贵资源。本试验旨在通过比较耐盐(NaCl)性不同的二倍体马铃薯在碱(NaHCO3)胁迫下的形态和生理性状的表现,探索二倍体马铃薯耐盐性和耐碱性之间是否有一定关系。试验材料包括15个富利亚(Solanum phureja,PHU)与窄刀薯(S.stenotomum,STN)杂种(PHU-STN)无性系(这15个无性系是根据耐盐性试验选出,分为高、中、低3组)及它们的亲本。在MS培养基中加入不同浓度的NaHCO3胁迫PHU-STN杂种无性系,处理28d后测定形态性状(芽长、芽鲜重、芽干重、根长、根鲜重和根干重)和生理性状(SOD活性、POD活性和丙二醛含量)。结果表明:二倍体马铃薯对碱敏感度与对盐敏感度基本一致,即耐盐二倍体马铃薯也耐碱,但由于绝大多数指标在三个组内的5个无性系间存在显著或极显著差异,说明这种关系并不是绝对的。

Potato （Solanum tuberosum L. ） is moderately sensitive to salt. Diploid potato has various genetic variation and is a valuable resource for salt and alkali-tolerance breeding. This study was conducted to investigate if there was any relationship between salt （NaC1） tolerance and alkali （NaHCO3 ） tolerance in diploid potatoes by comparing morphological and physiological traits of diploid potatoes with various levels of salt tolerance under alkali stress. Fifteen Solanum phureja （PHU） and S. stenotomum （STN） hybrid （PHU-STN） clones, which were selected and divided into three groups based on different salt tolerance determined by a salt tolerance test, and their two parents were stressed in MS medium with various concentrations of NaHCO3. Twenty-eight days after treatment, morphological traits （shoot length, shoot fresh weight, shoot dry weight, root length, root fresh weight, and root dry weight） and physiological traits （Superoxide dismutase （SOD） activity, Peroxidase （POD） activity and Malondialdehyde content） of the PHU-STN clones were determined. The results indicated that the sensitivity of diploid potatoes to NaC1 and NaHCO3 was generally consistent, namely, the salt-tolerant diploid potatoes were also tolerant to alkali. However, it should be noted that this relation was not absolute because the difference of the five clones in each of the three groups of various salt tolerances was significant or highly significant for most of the index tested under NaHCO3stress.