摘要
首次建立了高效液相色谱法同时测定葡萄酒中乙酰磺胺酸钾（安赛蜜）、糖精钠、苯甲酸、山梨酸含量的方法.样品水浴去除乙醇,用流动相甲醇＋乙酸铵（3：7,V/V）定容,采用PACKING FUJI SPS100 C18柱,（250×4.6mm,5μm）进行等度洗脱分离.乙酰磺胺酸钾、糖精钠、苯甲酸、山梨酸在1~100μg/mL范围内,线性关系良好,回归系数R2高于0.999 9,回收率88.4%~99.8%,精密度0.12%~1.84%,最低检出限均达到1.0μg/mL.方法准确、快速、灵敏度高,重现性好,满足食品中添加剂的检测要求,适用于葡萄酒中添加剂的分析检测,是葡萄酒质量监控强有力的技术支撑.
A method for the simultaneous determination of potassium acetyl- sulfamate,soluble saccharin,benzoic acid and sorbic acidin wine has been firstly developed using high performance liquid chromatography. After the removal of ethanol on a water bath,the sample was volumed with the methanol ＋ ammonium acetate（ 3： 7,V / V） mobile phase. The four analytes were eluted by isogradient on an PACKING FUJI SPS100 C18（ 250 × 4. 6 mm,5 μm） column using methanol and ammonium acetate solution as the mobile phase. Good linearities were achieved with R2≥0. 9999 for all analytes in the concentrations of 1. 0 to 100 μg/mL. The recoveries were in the range of 88. 4% to 99. 8% with RSD% in the range of 0. 12% to 1. 84 %. The detection limits of 4 analytes reached 1. 0 μg / mL. The method is accurate,rapid,sensitive,has good reproducibility and is suitable for the simultaneous determination of additives in wine. Moreover,the method can meet the requirements for the determination of additives in foods and can provide an alternative way for the wine quality monitoring.
出处
《分析测试技术与仪器》
CAS
2014年第1期31-37,共7页
Analysis and Testing Technology and Instruments
