白花曼陀罗植株再生及快繁体系的建立

Plant Regeneration and Clonal Propagation System of Datura metel

目的:探索白花曼陀罗植株再生与快繁体系的最佳培养条件。方法:以白花曼陀罗的茎段、叶片为外植体,建立以叶片为材料的植株再生体系和以茎段为材料的快繁体系。结果:白花曼陀罗不同外植体的最优消毒方法为:叶片采用75%酒精浸泡6 s,0.1%HgCl2浸泡6 min;茎段采用75%酒精浸泡8 s,0.1%HgCl2浸泡7 min。叶片的愈伤组织最佳培养基为MS+1.0 mg/L 6-BA+0.1 mg/L NAA,丛生芽的最佳培养基为MS+2.0 mg/L 6-BA+0.2 mg/L NAA;茎段的快繁体系最佳培养基为MS+3.0 mg/L 6-BA+0.05 mg/L NAA。组培苗生根的最佳培养基为MS+0.5 mg/L IBA;组培苗经驯化及移栽,成活率达90%以上。结论:建立了白花曼陀罗植株再生及快繁体系。

Objective：To study the condition of plant regeneration and clonal propagation system of Datura metel. Methods：Stems and leaves of Datura metel were used as explants, effects of different hormones for callus induction amt plant regeneration of leaves and clonal propagation system of stems were studied and optimized. Results：The optimal way to obtain sterile explant for leaves were steri- lized in 75% ethyl alcohol for 6 s then 0. 1% HgC12 for 6 rain;Stems were sterilized in 75% ethyl alcohol for 8 s then 0. 1% HgCI2 for 7 rain. The optimal medium for callus of leaves was MS ＋ 1.0 mg/L 6-BA ＋ 0. 1 mg/L NAA;The optimal medimn for callus induction of clustered buds was MS ＋2. 0 mg/L 6-BA ＋0. 2 mg/L NAA;The optimal medium for clonal propagation system of stems was MS ＋3.0 mg/L 6-BA ＋ 0, 05 mg/L NAA. The best medium for rooting induction was MS ＋ 0. 5 mg/L IBA. Transplant survival rate of plantlet was greater than 90% in humus soil-pearlite（5：1 ）. Conclusion：The condition of plant regeneration and clonal propagation system of Datura metel is established.