响应面法优化荭草花提取工艺研究

Studies on Optimizing Extraction Technics of Flowers of Polygonum orientale by Response Surface Methodology

目的:利用响应面分析法优化荭草花的最佳提取工艺。方法:以荭草花中花旗松素的含量为指标,选取乙醇浓度、提取时间、液料比3个因素进行Box-Behnken中心组合设计,采用响应面法对荭草花的提取工艺参数进行优化。结果:荭草花的最佳提取工艺为:乙醇浓度为65%,提取时间为129 min,液料比为18,在该条件下平均提取量为2.79 mg/g。结论:采用响应面法优化提取工艺合理,方法简单,且具有较好的预测性。

Objective： To analyze and optimize extraction technics of Polygonum orientale flowers by response surface methodology. Methods ： With the index for the content of taxifolin in flowers of Polygonum orientale, the effect of three factors such as concentration of alcohol,extraction time and solvent-solid ratio was designed by Box-Behnken central composite. Extraction technic pa- rameters of Polygonum orientale flowers was optimized by response surface methodology. Results ： The optimizing extraction conditions of Polygonum orientale flowers were as follows：ethanol concentration was 65% , extracting time was 129 rain and solvent-solid ratio was 18. Under the conditions,the average yield of taxifolin in 3 validation experiments was 2. 79 mg/g. Conclusion： Optimizing extraction technics by response surface methodology is reasonable, simple, and has good predictability.