沙柳原料高浓度底物酶解发酵乙醇工艺

Process of high-solid loadings on enzymatic hydrolysis and ethanol fermentation of Salix biomass

为了提高沙柳生物转化过程的经济可行性,考察了沙柳原料经过蒸爆、超微粉碎+稀酸、超微粉碎+稀碱预处理后高浓度底物补料酶解的效果,并对其高浓度水解糖液进行了乙醇发酵。结果表明:蒸爆处理法水解效果最好,通过补料酶解,底物质量分数可以达到30%,酶解液中总糖质量浓度达到132 g/L,葡萄糖质量浓度105 g/L;超微粉碎+稀酸预处理原料底物质量分数可以达到22%,酶解液中总糖质量浓度达到123 g/L,葡萄糖质量浓度73 g/L;超微粉碎+稀碱预处理原料底物质量分数可以达到22%,酶解液中总糖质量浓度133 g/L,葡萄糖质量浓度77 g/L。3种预处理使沙柳原料的酶解糖液都可以较好地被酿酒酵母利用发酵产乙醇,蒸爆处理原料的酶解糖液乙醇发酵效果最好,乙醇质量浓度达到47 g/L。

In order to improve the economic benefit of the Salix biorefinery process,this paper examined the Salix pretreatment by steam explosion,ultrafine grinding with dilute acid,ultrafine grinding with dilute alkali,followed by high solid concentration of enzymatic hydrolysis,and ethanol fermentation by yeast.The results showed that steam explosion is the best pretreatment method for Salix enzymatic hydrolysis.The substrate concentration could reach 30% by feeding enzymatic method.The enzymatic hydrolyzate of total sugar concentration was 132 g/L and the glucose was 105 g/L.When using the ultrafine grinding and dilute acid pretreated material,the substrate concentration could reach 22%, the enzymatic hydrolyzate of total sugar concentration was 123 g/L and glucose concentration was 73 g/L.When using the ultrafine grinding and dilute alkali pretreated material,the substrate concentration could reach 22%,the enzymatic hydrolyzate of total sugar concentration was 133 g/L and glucose concentration was 77 g/L.The enzymatic hydrolyzate of three pretreatment methods for Salix could be fermented to ethanol by yeast and the hydrolyzate of steam explosion pretreated materials could reach 47 g/L ethanol by fermentation.