摘要
Objective To observe effects of acupuncture prestimulus on heat shock protein HSP 70,HSP 90 and apoptotic rate of hippocampal neuron in model rats with Alzheimer＇s disease（AD） in order to investigate the functional mechanism in preventing and treating AD by acupuncture.Methods SD rats were randomly divided into control,sham operation,model and acupuncture prestimulus groups,with 10 rats in each group.In the pre-acupuncture group,electroacupuncture and moxibus on were given at ＂Bǎihuì＂（百会 GV 20） and ＂Shènshū＂（肾俞 BL 23） on both sides for three weeks,then Aβ1-42 was injected into hippocampus on both sides to prepare the model rats with AD. After AD＇s model was established,EA and moxibustion were given again for one week. Immunohistochemical analysis was carried out to determine the mean op cal density expression of HSP 70 and HSP 90 in CA 1 area of hippocampus in the rats,and flow cytometry was used to determine the apopto c rates of hippocampal neuron cells. Results A er acupuncture pres mulus for 4 weeks,the mean op cal density expression of HSP 70 and HSP 90 in CA 1 area of hippocampus in the rats significantly increased in comparison to that in the model group（P0.01）,and the apopto c rate of the cells significantly decreased in comparison to that in the model group（P0.01）. Conclusion Acupuncture pres mulus can inhibit cell apoptosis by inducing the expression of HSP 70 and HSP 90 in brain,and thus protect neurons.
目的:通过观察针灸预刺激对阿尔茨海默病(AD)模型大鼠热休克蛋白HSP 70、HSP 90及海马神经元凋亡率的影响,探讨针灸防治AD的作用机制。方法:将SD大鼠随机分为4组,即正常组、假手术组、模型组、预针灸组,每组各10只,取"百会"和双侧"肾俞"穴电针并温和灸刺激3周后,从双侧海马注入Aβ1-42制作AD大鼠模型,然后再电针和艾灸刺激1周。采用免疫组化法测定大鼠海马CA 1区HSP70、HSP 90的平均吸光度值,流式细胞仪检测各组大鼠海马神经元细胞凋亡率。结果:通过4周的预针灸刺激,大鼠海马CA 1区HSP 70、HSP 90的平均吸光度值均较模型组有显著提高(P<0.01),细胞凋亡率较模型组显著降低(P<0.01)。结论:针灸预刺激能通过诱导脑内HSP 70、HSP 90的表达来抑制细胞凋亡现象,从而起到保护神经元的作用。
基金
Supported by National Natural Science Founda on of China:81173323,30901925
Natural Science Founda on of Hubei Province:2007 ABA 084
