连花清瘟颗粒／胶囊治疗甲型H1N1流感作用机理的网络生物学分析

Biological Network Analysis on Mechanism of Lianhua Qingwen Granules /Capsules for H1N1 Influenza A

目的采用网络生物学方法探讨连花清瘟颗粒/胶囊治疗甲型H1N1流感的药理机制。方法用文本挖掘文献证实甲型H1N1流感相关的基因或蛋白质,查询PubChem数据库获得连花清瘟颗粒/胶囊的靶蛋白,依据多个相互作用数据库中数据,分别构建甲型H1N1流感体内反应的蛋白质相互作用网络和连花清瘟颗粒/胶囊的靶蛋白相互作用网络,建立连花清瘟颗粒/胶囊对抗甲型H1N1流感体的靶蛋白相互作用网络,并进行网络拓扑结构和基因本位论(GO)分析。结果连花清瘟颗粒/胶囊药理作用主要涉及细胞对外部刺激反应的抑制性调节、细胞凋亡的调节和信号转导等方面,主要涉及蛋白激酶B(AKT1)、胱天蛋白酶3(CASP3)、丝裂原活化蛋白激酶1(MAPK1)、肿瘤基因P53(TP53)、核因子-κBp65(RELA)、核因子-κBp50(NFKB1)、热休克蛋白90-alpha(HSP90AA1)等。结论连花清瘟颗粒/胶囊可能主要是通过影响AKT1、CASP3等信号通路,调节细胞凋亡而减少病毒复制,抑制甲型H1N1流感的疾病进程。

Objective To study the pharmacological mechanism of Lianhua Qingwen Granules^Capsules for H1N1 influenza A by biological network method. Methods The H1N1 influenza-related genes or proteins were confirmed by text mining. The target proteins of Lianhua Qingwen Granules^Capsules were obtained by searching the PubChem Database. The protein interaction networks of H1 N1 influenza A and target protein interaction networks of Lianhua Qingwen Granules/Capsules were constructed and the latter was mapped to the former to establish the target protein interaction networks of Lianhua Qingwen Granules^Capsules against HI N1 influenza. The network topology and gene ontology （GO） analysis were conducted. Results The pharmacological effect of Lianhua Qingwen Granules/ Capsules was mainly related to inhibitory regulation of cells to external stimulation, regulation of apoptosis and signal transduction. The protein kinase B （ AKT1 ）, caspase 3 （ CASP3 ）, mitogen-activated protein kinase 1 （ MAPK1 ）, tumor gene p53 （TP53）, nuclear factor-κB p65 （RELA）, nuclear factor-κB p50 （NFKBI）, and heat shock protein 90-alpha （HSP90AA1） was involved. Conclusion Lianhua Qingwen Granules/Capsules can inhibit the disease progression of H1 N1 influenza A possibly by affecting AKT1 and CASP3 signaling pathways, regulating apoptosis, and reducing viral replication.