PEG干旱胁迫对大花飞燕草幼苗生理特性的影响

Physiological characteristics of Delphinium grandiflorum under drought stress

为研究大花飞燕草(Delphinium grandiflorum)幼苗抗旱性,采用不同浓度(0、5%、10%、15%、20%和25%)的PEG溶液对大花飞燕草幼苗进行模拟干旱胁迫,测定了其细胞膜透性、丙二醛、脯氨酸、过氧化物酶、超氧化物歧化酶以及可溶性蛋白等生理生化指标。结果表明,随着干旱胁迫的加剧,大花飞燕草幼苗叶片细胞膜透性、丙二醛含量和脯氨酸总体呈上升趋势;超氧化物歧化酶和过氧化物酶活性总体呈先升后降的趋势,可溶性蛋白在各个胁迫条件下差异不大;综合各指标表明大花飞燕草幼苗具有一定的耐旱性。

The experiment was conducted to estimate the drought tolerance of Delphinium grandiflorum seedling stressed by PEG with the concentrations of 0, 5%, 10%, 15%, 20% and 25%, respectively. Different physiology indices including cell membrane permeability, malondialdehyde （MDA）, free proline （Pro）, peroxidase （POD） activity, superoxide dismutase （SOD） activity and soluble protein were measured. The results showed that the cell membrane permeability, MDA and Pro concentration in seedling leaves of D.grandiflorum increased with drought stress increased. SOD and POD activity increased firstly and then decreased with drought stress increased. Soluble protein concentration had no difference between control and different stress treatments. All of these variation suggested that the seedling of D.grandiflorum had drought tolerance in some degree.