血卟啉单甲醚声动力作用对兔耳增生性瘢痕成纤维细胞的急性细胞毒作用

Acute toxicity in fibroblast histomorphology of rabbit ear induced by sonodynamic therapy with hematoporphyrin monomethyl ether

目的观察血卟啉单甲醚（HMME）声动力作用对兔耳增生性瘢痕成纤维细胞的急性细胞毒作用。方法首先采用噻唑蓝（MTF）法检测不同超声辐照时间及不同HMME浓度对兔耳增生性瘢痕成纤维细胞存活率的影响，以确定最佳辐照时间和HMME浓度。将兔耳增生性瘢痕成纤维细胞悬液分为4组，以M1Tr法检测各组细胞的存活率，膜联蛋白V（AnnexinV）/碘化丙锭（PI）双染色经流式细胞技术检测其死亡方式。结果超声辐照30、60、90s时，可明显降低兔耳增生性瘢痕成纤维细胞的存活率（P〈0．05），辐照时间为10S时，对细胞存活率无明显影响（P〉0．05）；单纯HMME对细胞存活率无明显影响（P〉0．05）。当辐照时间为10s，HMME为20mg/L和50mg/L时，细胞存活率明显下降（P〈0．05），AnnexinV/PI双染色检测结果显示兔耳增生性瘢痕成纤维细胞存在坏死与凋亡。结论HMME是一种有效的声敏剂，其声动力效应对体外培养兔耳增生性瘢痕成纤维细胞具有显著杀伤作用。

Objective The aims of the present study were to observe hematoporphyrin monomethyl ether （HMME） as a sonosensitizer and investigate the acute toxicity in fibroblast histomorphology of rabbit ear induced by sonodynamic therapy with hematoporphyrin monomethyl ether （HMME-SDT）. Methods The effects of various ultrasound irradiation time and various concentrations of HMME on the survival of fi- broblast were determined by methyl thiazol tetrazolium （MTT）. To definite iIradiation time and concentra tion of HMME. The cells were divided four groups. The Acute toxicity was observed by MTF. The death modality was investigated by flowcytometry. Results There was a significant difference （P 〈 0. 05 ） when the irradiation time were 30, 60, 90 s. However, there were no significant differences （P 〉 0. 05 ） for HMME. The irradiation time： 10 s, HMME： 20 mg/L, the survival of the HMME-SDT group was signifi cantly lower than the C, HMME and U groups （ P 〈 0. 05）. Necrosis and apoptosis be observed by flowcy tometry. Conclusion HMME is an effective sonosensitizer. HMME-SDT can significantly kill fibroblast histomorphology of rabbit ear, there were necrosis and apoptosis.