前列腺癌相关成纤维细胞中雄激素受体促进前列腺癌侵袭转移能力的研究

The androgen receptor in cancer-associated fibroblasts could promote the invasive abilities of prostate cancer

目的观察前列腺癌（PCa）相关成纤维细胞（CAF）中雄激素受体（AR）对PCa侵袭转移能力的作用。方法原代培养并永生化CAF细胞，用RNA干扰方法降低CAF细胞AR表达，得到CAF-ARsi细胞及对照组细胞，并用Westernblot方法检测AR表达水平。将上述细胞与PCa的PC3细胞体外共培养，用Transwell侵袭实验检测PC3侵袭能力，用软琼脂糖凝胶实验检测PC3细胞悬浮生长能力。结果成功建立CAF-ARsi细胞，Westernblot结果显示CAF-ARsi细胞中AR表达水平显著低于CAF-se细胞。PC3细胞与CAF-ARsi共培养后，其侵袭能力低于对照组细胞（0．23±0．03比0．39±0．04，P〈0．01），悬浮生长能力减弱[（18．9±1．7）个比（28．5±2．6）个，P〈0．01]。结论CAF细胞中AR对PCa细胞的侵袭转移能力起重要作用。

Objective To study the roles of androgen receptor in cancer-associated fibroblasts （CAF） on the invasive abilities of prostate cancer. Methods The CAF cells were cultured and immortal ized by the SV40-T. The androgen receptor （AR） in CAF was knocked down by SiRNA. The prostate cancer cell line PC3 was co-cultured with resultant cells （ CAF-ARsi or CAF-sc）. The transwell and colony formation assay were used to test the invasive ability and anchor-independent growth ability. Results The CAF cells were successfully cultured and immortalized. In the transwell assay, the PC3 cells co-cultured with CAF-ARsi cells had fewer invading cells than control cells （ 0. 23 ± 0. 03 vs. 0. 39 ± 0. 04, P 〈 0. 01 ）. In colony formation assay, the PC3 cells co-cultured with CAF-ARsi cells formed smaller and fewer colonies （ 18.9 ± 1.7 vs. 28.5 ± 2. 6, P 〈 0. 01 ）. Conclusion The AR in CAF cells could promote the invasive abilities of prostate cancer. Targeting the AR in CAF cells might be a potential therapeutic option for prostate cancer in future.