同种异体肋软骨细胞修复兔膝关节全层软骨缺损的研究

Aliogeneic costal chondrocyte to repair the full-thickness cartilage defect on the knee of the rabbitmodel

目的观察同种异体肋软骨细胞复合透明质酸钠对兔膝关节全层软骨缺损的修复作用。方法分离、培养肋软骨细胞，鉴定生物学活性。将36只新西兰大白兔制备膝关节全层软骨缺损模型并随机分为3组（n=12）。空白组不进行特殊处理；对照组注入透明质酸钠；实验组注入肋软骨细胞／透明质酸钠复合物。术后第1、2、3个月各组分别随机处死4只兔并获取股骨远端标本，行肉眼大体观察、病理组织学检测，按0’Driseoll，keeleyandsalter法行组织学评分。结果第2代肋软骨细胞阿尔辛蓝、番红O、Ⅱ型胶原染色均呈强阳性。实验组术后第1、2、3个月组织学评分（8．75000±0．50000、14．00000±0．81650、19．00000±0．81650）明显高于对照组（0．75000±0．50000、2．00000±0．81650、5．00000±0．81650）和空白组（0．50000±0．57735、0、0），同一时间点实验组与对照组和空白组组织学评分比较差异均有统计学意义（P〈0．05）。结论第2代肋软骨细胞适合作为软骨组织工程的种子细胞；肋软骨细胞复合透明质酸钠修复兔膝关节全层软骨缺损近期效果满意。

Objective To explore the effects of allogeneic composites of costal chondrocyte/Sodi um hyaluronate in repairing the full-thickness cartilage defects at knee joints of rabbit models. Methods Condrocytes were isolated and cultivated for evalutating biological activities. Full-thickness cartilage defect models from knee joints of 36 Newzealand rabbits were randomly and evenly allocated into blank, control and experimental groups. The blank group was subjected to no disposal. For control group, sodium hyalur onate was injected into the defect region, and costal chondrocytes/sodium hyaluronate composites were injected into the defect region in experimental group. Every 4 rabbits were executed ramlomly by the end of the first, second and third month respectively for specimens sampled from distal femur and the specimens were obsevered under direct vision, examined by histdogical staining and scored according to O＇ Driseoll, keeley and salter scales respectively. Results The 2nd generation costal ehondrocytes were remarkably stained by alcian blue, sarranine O and collagen （ type II ） respectively. The histological scores of experi- mental group （ 8. 750 00 ± 0. 500 00, 14. 000 00 ± 0. 816 50, 19. 000 00 ±0. 816 50 ） were significantly higher than those of blank group （0. 750 00 ±0. 500 00,2. 000 00 ±0. 816 50,5. 000 00 ±0. 816 50） and blank group （0. 500 00 ± 0. 577 35, 0, 0） based upon statistical differences （P 〈 0. 05 ）. Conclusion Costal chondroeytes of the 2nd genretation is qualified as seed cells for cartilage tissue engineering re- search. Allogeneic costal chondrocyte/sodium hyaluronate composites contribute to restore full-thickness cartilage defect at knee joints of the rabbit models satisfactorily.