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CD4^+T细胞三磷酸腺苷值及T细胞亚群测定在肝移植术后免疫功能监测中的应用

Application of adenosine-triphosphate value of CD4 ^+ T cells and T cell subsets analysis in monitoring immune function in post-liver transplantation recipients
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摘要 目的探讨CD4^+T细胞三磷酸腺苷(ATP)值及T淋巴细胞亚群测定在肝移植术后免疫功能监测中的应用价值。方法采集48例行肝移植术后患者和30例健康志愿者的外周血样本,采用Irnmuknow免疫细胞功能测定试剂盒,检测CD4^+T细胞ATP值;流式细胞仪检测外周血T淋巴细胞亚群CD3^+、CD4^+、CD8^+细胞的比例。结果正常人、稳定组、感染组与急性排斥组ATP值分别为(331±103)、(364±125)、(182±104)和(479±116)μg/L,急性排斥组明显高于感染组(P〈0.01);稳定组与感染组比较,感染组明显降低(P〈0.01);稳定组与急性排斥组比较差异无统计学意义(P〉0.05)。T淋巴细胞亚群检测结果,稳定组CD4^+细胞百分比为(33.23±11.34)%,感染组为(25.53±6.89)%,两组比较差异有统计学意义(P〈0.05)。结论CD4^+T细胞ATP值以及T淋巴细胞亚群CD4^+细胞百分比能较好地反映肝移植患者术后免疫功能。 Objective To investigate the significance of adenosine-triphosphate (ATP) value of CD4 ~ T cells and T cell subset analysis in monitoring immune function after liver transplantation. Methods Immuknow immune cell function kit was applied to test the ATP value of CD4 ^+ T cells in peripheral blood from 48 patients undergoing liver transplantation and 30 healthy volunteers. Additionally, the frequency of CD3 ^+ , CD4^+and CD8 ^+ cell subsets was analyzed hy flow cytometry. Results For healthy volunteers, stable recipients, infectious recipients and recipients with acute rejection, the mean ATP levels were (331 ± 103), (364 ± 125), ( 182 ± 104) and (479± 116) μg/L, respectively. The ATP levels in acute rejection patients were higher than stable ones (P 〈0. 01 ) and patients with infections (P 〈0. 01 ). How ever, there was no statistically significant difference between stable recipients and recipients with acute re jection (P 〉 0. 05). T cell subsets analysis showed that the CD4 ^+ cell counts were (25.53± 6.89)% in the infectious patients and ( 33.23 ± 11.34 ) % in stable group, there was a significantly different in two groups ( P 〈 0. 05 ). Conclusion The ATP levels in CD4 ^+ T cells and T cell subsets analysis can indicate the immune status after liver transplantation to a certain extent.
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2014年第4期843-845,共3页 Chinese Journal of Experimental Surgery
基金 国家自然科学基金资助项目(81370574) 国家高技术研究发展计划(863计划)资助项目(2012AA021008) 高等学校博士学科点专项科研基金资助项目(博导类)(20120171110073) 广东省自然科学基金资助项目($2012010009376) 广东省科技计划资助项目(20108031600222)
关键词 肝移植 CD4^+T细胞 淋巴细胞亚群 免疫 Liver transplantation CD4^ + T cell Lymphocyte subsets Immune
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