趋化因子受体4在胆管癌组织中的表达及其临床意义

Expression of CXC chemokine recetper 4 and its clinical significance in cholangiocarcinoma

目的观察CXC趋化因子受体-4（CXCR4）在胆管癌中的表达，探讨CXCR4与胆管癌临床病理特征及预后的关系。方法采用免疫组织化学染色及逆转录一聚合酶链反应（RT-PCR）法观察CXCR4在82例胆管癌、7例胆道良性肿瘤、12例非肿瘤胆道上皮组织以及30例新鲜胆管癌组织中的表达。结果CXCR4蛋白在82例胆管癌、7例胆道良性肿瘤、12例非肿瘤胆道上皮组织中阳性表达率分别为75．6％、28．6％和0。CXCR4蛋白在胆管癌组织中的表达水平明显高于胆道良性肿瘤和非肿瘤胆道上皮组织（P〈0．叭）。胆管癌组织中CXCR4蛋白表达Ⅱ～Ⅳ期患者均高于I期的表达（22．4％比7．3％，P〉0．05）。RT-PCR结果显示胆管癌组织中CXCR4mRNA相对表达水平与淋巴结转移和临床分期密切相关，淋巴结转移组和非转移组的相对表达量分别为0．44±0．07和0．23±0．03，淋巴结转移组明显高于非转移组（P〈0．05）；Ⅱ、Ⅲ期病例CXCR4mRNA的表达水平明显高于I期病例（0．434-0．07比0．25±0．08，P〈0．05）。结论CXCR4可能参与了胆管癌的发生，并促进胆管癌的浸润、转移。

Objective To investigate the relationship between CXC chemokine recetper 4 （CXCR4） expression to elinieopathological factors and prognosis in cholangiocarcinoma. Methods The expression of CXCR4 in 82 cases of cholangiocareinoma, 7 cases of benign bile duct neoplasms and 12 ca- ses of non-tumor bile duct epithelium was detected by immunohistoehemical staining, and that in 30 cases of cholangiocarcinoma by reverse transcriptase-polymerase chain reaction （RT-PCR）. Results The posi tive expression rate of CXCR4 in 82 cases of cholangiocarcinoma, 7 cases of benign bile duct neoplasms, and 12 cases of non-tumor bile duct epithelium was 75.6% , 28.6% and 0, respectively. The expression of CXCR4 in cholangiocarcinoma was significantly higher than that in benign bile duct neoplasms and non tumor bile duct epithelium （ P 〈0.0l ）. The positive rate of CXCR4 was slightly higher in the stage Ⅱ -Ⅳ cases than that in the stage I cases （22.4% vs. 7.3% ,P 〉0. 05）. RT-PCR results revealed that the ex pression of CXCR4 mRNA in stage Ⅱ and Ⅲ cases was higher than that in stage I cases （0.43 ±0. 07 vs. O. 25 ± 0. 08, P 〈 O. 05 ）. The expression of CXCR4 mRNA in the cases with lymph node metastasis was significantly higher than that without lymph node metastasis （ 0. 44 ± 0. 07 vs. 0. 23 ± 0. 03, P 〈 0. 05 ）. Conclusion CXCR4 may play a positive role in carcinogenesis, invasion and metastasis of cholan giocarcinoma.