血清白细胞介素-17水平对类风湿性关节炎患者骨关节损害作用及机制

Serum interleukin-17 levels and rheumatoid arthritis patients with bone and joint damage

目的探讨血清白细胞介素（IL）-17水平对类风湿性关节炎（RA）患者骨关节损害作用及机制。方法我院收治的RA患者55例作为实验组，选取健康人55例作为对照组。检测两组血清IL-17和抗环瓜氨酸多肽抗体（抗CCP）水平，对比不同x线分期的RA患者血清IL-17水平，并观察患者活动期、类风湿因子和晨僵时间与患者血清IL-17水平的关系。结果实验组患者IL-17水平[（308．42±76．14）ng、L]和抗CCP水平[（1325．3±234．1）AU/mL]显著高于健康对照组[（124．38±48．65）ng/L、（24．6±11．7）AU/ml]，差异有统计学意义（P〈0．05）；实验组患者X线分期越高，m清IL-17水平越高[期的血清IL-17水平分别为（208．23±67．41）、（322．62±78．35）和（452．81±88．32）ng/L]，差异有统计学意义（P〈0．05）；处于活动期的患者血清IL-17水平[（342．61±101．41）ng/L]显著高于非活动期的患者[（228．61±101．54）ng/L]，类风湿因子阳性的患者血清IL-17水平[（318．74±98．42）ng/L]高于类风湿因子阴性的患者[（220．56±99．62）ng/L]，晨僵时间长的患者血清IL-17水平[（356．27±88．74）ng/L]显著高于晨僵时间短的患者[（201．38±78．43）ng/L]，差异有统计学意义（P〈0．05）。结论血清IL-17在RA的发生发展中起重要作用，骨关节损害严重的患者血清IL-17水平高。

Objective To observe the correlation between serum interleukin （IL）-17 levels with bone and joint damage in rheumatoid arthritis （RA） patients and the possible mechanism. Methods Fifty five cases of RA in our hospital were selected as the observation group during the period from October 2010 to June 2013, and 55 healthy volunteers were selected as the control group simultaneously. The serum IL-17 and anti-cyclic citrullinated peptide antibody （anti-CCP） levels were detected by double antibody sandwich enzyme linked immunosorbent assay （ELISA）. The serum IL-17 levels in RA patients with different stages of X-ray were compared. The relationship between serum IL-17 levels with active phase, rheumatoid factor and morning stiffness was observed. Results The serum IL-17 levels [ （308.42 ± 76. 14） ng/L] and anti-CCP levels [ （ 1 325.3 ±234. 1 ） AU/ml] in the observation group were significantly higher than those of the con trol group （P 〈0. 05）. There was significant difference in the serum IL-17 levels among different stages of X-ray. The higher the stages of X-ray, the higher the levels of serum IL-17 [ the serum IL-17 levels at stage Ⅰ, ⅡandⅢ： [（208.23±67.41）, （322.62±78.35）, （452.81±88.32） ng/L, P〈0.05]. The serum IL-17 levels at the active phase [ （342. 61 ± 101.41 ） ng/L]were significantly higher than those of the patients at the nonactive period [ （228.61 ± 101.54） ng/L]. The serum IL-17 levels in patients positive for serum rheumatoid factor [ （318. 74 ± 98.42） ng/L 1 were higher than those of the patients negative for serum rheumatoid factor-negative [ （220. 56 ±99. 62） ng/L]. The serum IL-17 levels were significantly higher in patients with long duration of morning stiffness [ （356. 27 ±88.74） ng/L] than those in patients with a short duration of morning stiffness [ （201.38 ± 78.43） ng/L, P 〈 0. 05 ]. Conclusion Serum IL-17 plays an important role in the development of RA. Patients with severe bone and joint damage have high serum IL-17 levels.