局部灌注药物保护缺血脊髓微创模型的改建

Modification of the minimally invasive model for regional drug protection against spinal cord ischemia-reperfusion injury in rabbits

目的建立兔腹主动脉局部灌药保护缺血脊髓微创模型。方法对照钳夹法，采用不同球囊导管阻断腹主动脉，比较脊髓损伤复制效果；缺血期间经腹主动脉灌注利多卡因40mg/kg，比较药物保护效果。结果4FSwan-Ganz球囊导管联合PC导管法与钳夹法截瘫率均为87．5％，脊髓前角正常神经元计数分别为0（0～1．75）个和1（0-2．00）个。灌注利多卡因时，球囊导管法与钳夹法截瘫率分别为25．0％和12．5％，前角正常神经元计数分别为8．5（2．25～12．00）个和9．0（4．75～10．75）个，两种建模方法差异无统计学意义（P〉0．05）。结论4FSwan．Ganz球囊导管联合PC导管可替代传统动脉夹阻断兔腹主动脉，建立局部灌药保护缺血脊髓微创模型。

Objective To establish a minimally invasive model for regional aorta-infused drug pro tection against spinal cord ischemia-reperfusion injury in rabbits. Methods Spinal cord ischemia was in duced in New Zealand rabbits by infrarenal abdominal aorta occlusion with different balloon catheters or an artery clamp for 30 vain, During ischemia, normal saline or [idocaine 40 mg/kg was consecutively infused in to the occluded aortic segments via a polycarhonate catheter through the femoral artery. Thereafter, blood flow was regained for reperfusion. Neurological function was assessed according to Tarlov scales postopera tively. IA-5 segements of the spinal cord were harvested at 48 h after reperfusion for histopathologic evalua tion, and the total number of normal motor neurons in the anterior horn was counted. Results The 4F Swan- Ganz balloon catheter combined with a polycarbonate catheter was appropriate for modeling. Tarlov scores, the incidence of paraplegia （87. 5% in normal saline groups; 25.0% vs. 12. 5% in lidocaine groups） and the number of normal motor neurons [0 （0-1.75） vs. 1 （0-2.00） in normal saline groups; 8.5 （2.25- 12. 00） vs. 9.0 （4. 75-10. 75） in lidocaine groups] were similar between balloon ocelusion and aorta clam ping while the same solution was infused （ P 〉 0. 05 ）. Conclusion The 4F Swan-Ganz balloon catheter com- bined with a polycarbonate catheter can be used to establish the minimally invasive model for regional drug protection against spinal cord ischemia-reperfusion instead of conventional aorta clamping in rabbits.