摘要
目的采用HPLC法同时测定白喉乌头中氢溴酸高乌甲素及乌头碱、新乌头碱的量。方法采用XBridgeTMC18生物碱专用色谱柱(250 mm×4.6 mm,5μm),以0.05%冰醋酸(三乙胺,调pH为7.25)-乙腈为流动相梯度洗脱,体积流量1 mL/min,柱温30℃,检测波长为235 nm,进样量10μL。结果白喉乌头中同时含有氢溴酸高乌甲素、乌头碱、新乌头碱生物碱类成分,其中氢溴酸高乌甲素的量相对较高,有一定的应用开发前景。结论本办法结果可靠,操作简便,稳定性好。
AIM To determine the contents of hydrobromide lappaconitine and aconitine and new aconitine in Aconitum leucostomum Worosch by HPLC. METHODS XBridge C18 alkaloid dedicated chromatographic column （ 250 mmx 4. 6 mm, 5 μm） was employed with 0. 05% （ pH7.25 of glacial acetic acid and triethylamine） - ace tonitrile as solvent system in gradient elution mode. The flow rate was 1.0 mL/min, column temperature was 30 ℃ , detection wavelength was set at 235 nm, and injection size was 10 μL. RESULTS Aconitum leucosto mum Worosch contained hydrobromide lappaconitine, aconitine and new aconitine, the former hydrobromide lap paconitine content was relatively high. CONCLUSION The method is reliable, stable and easy to operate.
出处
《中成药》
CAS
CSCD
北大核心
2014年第4期796-799,共4页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(81160498)
