肝母细胞瘤中的干细胞表面标记物的初步识别

Preliminary recognition of stem cells surface markers in hepatoblastoma

目的初步筛选出肝母细胞瘤(HB)中的肿瘤干细胞(NSC)相关表面标记组合,并了解NSC的分布。方法选择入住江西省儿童医院并接受手术的HB患儿,采用免疫组织化学染色的方法,观察干细胞相关标记物CD34、Thy-1、c-kit、CD56和干细胞生长因子(SCF),在HB组织及距肿瘤组织边缘3cm以外的正常肝脏组织的表达和分布。结果 Thy-1、c-kit散在分布于HB组织中,主要集中在汇管区,而正常肝脏组织中不表达;CD34及SCF在HB中的表达明显高于正常肝脏组织(P<0.05),其中CD34主要在血管内皮系统中分布,SCF主要表达在汇管区;CD56表达于成簇的神经纤维组织中,在HB及正常肝脏组织中表达差异无统计学意义(P>0.05)。结论不同干细胞相关表面标志物分布于HB组织中,并且集中表达在特定区域。表达Thy-1/c-kit阳性细胞可能对HB的发生起一定作用。

Objective To preliminarily screen the neoplastic stem cell （NSC） related surface markers combination and to under-stand the NSC distribution in hepatoblastoma（HB） .Methods The children cases of HB undergone the surgical therapy in the Jian-gxi Provincial Children′s Hospital were selected .The immunohistochemical method was adopted for observing the expression and distribution of NSC-related markers CD34 ,Thy-1 ,c-kit ,CD56 and stem cell factor（SCF） in the HB tissue and the normal hepatic tissue away from the edge of tumor tissue 3cm outside .Results Thy-1 and c-kit were sporadically distributed in the HB tissue and mainly focued on the portal area ,but did not exressed in the normal liver tissue ;the expression of CD34 and SCF in HB was signifi-cantly higher than that in the normal liver tissue（P＆lt;0 .05） ,in which CD34 was mainly distributed in the endothelial system and SCF was mainly expressed in the portal area ;CD56 was expressed in the tissue of the clustered nerve fibers ,the expression differ-ence between them had no statistical significance （P＆gt;0 .05） .Conclusion The different NSC related surface markers are distributed in the HB tissue and focus on the specific areas .The positive cells of Thy-1/c-kit expression may play a role in the HB occurrence .