马尾松种子萌发期的内源激素和酶活性变化

Variation of Endogenous Hormone and Enzyme Activity in Pinus massoniana Seeds During Germination Stage

探明马尾松种子的萌发机理,为贵州马尾松种子育苗提供参考,采用酶联免疫间接法对马尾松种子萌发期胚乳内的GA3、IAA、ABA、ZR、SOD、POD和淀粉酶含量进行测定,研究马尾松种子萌发过程中各种内源激素和酶活性的变化。结果表明:1)在马尾松种子萌发期,胚乳内的GA3、IAA、SOD、POD和淀粉酶含量随种子萌发时间的延长逐渐升高,0h到80h时的增幅分别为61.7%、93.6%、48.3%、49.5%和26.7%;ZR含量呈先升高后降低变化趋势,并在60h时达最高,为30.29ng/g;ABA含量呈逐渐降低趋势,由0h时的48.13ng/g降到80h时的24.24ng/g。2)马尾松种子胚乳中的GA3/ABA、IAA/ABA和ZR/ABA随种子萌发培养时间的延长呈逐渐升高趋势,并在培养结束时达到最大值;GA3、IAA、SOD、POD、淀粉酶含量的升高有助于马尾松种子萌发,ABA含量的升高则抑制马尾松种子萌发。因此,协调好马尾松种子各内源激素的变化对其种子顺利萌发有重要作用。

To discuss the mechanism of P .massoniana seed during germination stage and provide references for cultivating P .massoniana seedlings,the changes of GA3 ,IAA,ABA,ZR contents and SOD,POD and amylase were studied by indirect ELISA method.Results：1）GA3 ,IAA,SOD,POD and amylase presented an gradually increasing trend in initial phase,with the growing rates of 61.7%, 93.6%,48.3%,49.5% and 26.7% respectively.ZR first increased and then decreased,and reach the maximum of 30.29 ng/g at 60 h.ABA gradually decreased from 48.13 ng/g to 24.24 ng/g.2）Values of GA3/ABA,IAA/ABA and ZR/ABA gradually increased with culture time prolonging and reached the maximum in the end of cultivation.The enhancement of GA3 ,IAA,SOD,POD and amylase were beneficial for P .massoniana seed germination.While the enhancement of ABA inhibited the germination. So the coordination of various endogenous hormones played an important role in seed germination.