野桑蚕丝氨酸蛋白酶样蛋白基因克隆及特征(英文)

Cloning and characterization of Bombyx mandarinaserine protease-like protein

利用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)克隆野桑蚕(Bombyx mandarina)丝氨酸蛋白酶样蛋白基因,获得的cDNA序列长为938bp,含有一个912bp的完整开放阅读框,编码的蛋白质含有303个氨基酸残基.该基因编码的氨基酸序列与其他昆虫丝氨酸蛋白酶基因相应的氨基酸序列有较高的同源性,该序列具有它们丝氨酸蛋白酶基因家族的典型特征.RT-PCR和Northern blot检测分析表明该基因仅在野桑蚕5龄3d幼虫的血液中表达.这为进一步研究该基因的功能奠定了基础.

The complementary deoxyribonucleic acid（cDNA）of Bombyx mandarina serine protease-like protein gene was cloned by reverse transcription-polymerase chain reaction （RT-PCR）.The results showed that the cDNA with 938 bp in length contained an open reading frame（ORF）of 912 bp which encoded 303 amino acid residues.The encoded amino acid sequence had a high identity to the reported sequence of serine protease-like protein from other insects and shared the typical structural features of serine protease family.The serine protease-like protein gene was only expressed in haemolymph of B.mandarina by RT-PCR and Northern blot analysis. These results provide a molecular basis for further studying the function of this gene in B.mandarina .