高效液相色谱法同时测定拉莫三嗪、卡马西平、苯巴比妥的血药浓度

Simultaneous Determination of Lamotrigine,Carbamazepine and Phenobarbital in Human Plasma by High-Performance Liquid Chromatography

目的：建立同时测定拉莫三嗪（LTG）、卡马西平（CBZ）、苯巴比妥（PB）血药浓度的高效液相色谱（HPLC）法。方法：色谱柱：SunFire^TM C18（250mm×4．6mm，5μm）；流动相：水-乙腈-三乙胺（64．5：35：0．5），磷酸调节pH6．5；流速1．0mL／min，检测波长237nm；血浆样品经乙酸乙酯-二氯甲烷（75：25）提取，以布洛芬为内标。结果：LTG、CBZ在0．625～40．000Fg／mL、PB在1．5625～100．0000μg／mL的血药浓度范围内，其相应峰面积和内标峰面积的比值与浓度呈良好线性关系，相对回收率91％～101％，日内和日间RSD均〈8％。结论：该方法专属性强、准确灵敏、操作简便，适用于LTG、CBZ、PB血药浓度监测和药动学研究。

Objective： To establish a simultaneous determination method of lamotrigine, carbamazepine, and phenobarbital in human plasma by high-performance liquid chromatography. Methods： The analytical column was packed with SunFire^TM C18 （250 mm×4.6 mm, 5 μm）. An isocratic elution was applied at a flow rate of 1.0 mL/min with a mobile phase composed of water-acetonitrile-TEA （64.5 ： 35 ： 0. 5 ） adjusted at pH 6.5 with phosphoric acid. The wavelength detection was set at 237 nm. Plasma samples were extracted with ethylacetate-dichloromethane （75 ： 25 ）. Results： Calibration curves were linear with regression coefficients greater than 0. 9991 over the concentration ranges 0.625 - 40.000 μg/mL for LTG and CBZ, 1. 562 5 - 100. 000 0 μg/mL for PB, and the relative recovery was 91% - 101%. Both the relative standard deviations of interday and intraday were less than 8%. Conclusions： This bioanalytical method is rapid, sensitive and suitable for clinical therapeutic drug monitoring and also for supporting other clinical pharmacokinetic -based studies.