非细菌性前列腺炎大鼠L6-S1节段脊髓星形胶质细胞的活化状态研究

Activation of astrocytes in the L6-S1 spinal cord in nonbacterial prostatitis rat model

目的 观察化学性刺激大鼠前列腺后相应节段脊髓星形胶质细胞的活化状态,探讨脊髓星形胶质细胞是否参与了前列腺炎性疼痛的形成过程.方法 2011年3月至2012年3月选取24只雄性SD大鼠,随机取6只作为假手术组,余18只于前列腺内注射弗氏完全佐剂制备非细菌性前列腺炎模型,然后再随机分为建模3、7、10d组,每组6只.采用HE染色光学显微镜下观察前列腺组织腺腔形态、血管扩张程度及炎症细胞浸润程度,免疫荧光组化染色并结合Imagepro-Plus 6.0图像测量软件检测大鼠L6-S1节段脊髓星形胶质细胞胶质纤维酸性蛋白表达的累积吸光度变化.结果 HE染色观察3d组炎症反应轻微（血管轻度扩张充血,血管周围和间质内可见少量的中性粒细胞和淋巴细胞浸润）,7d组炎症反应强烈（血管扩张充血严重且破裂,血管周围和间质内可见大量的中性粒细胞和淋巴细胞浸润）,10d组炎症反应与7d组基本一致.免疫荧光染色观察各组大鼠脊髓组织胶质纤维酸性蛋白表达的累积吸光度,3d组为24 805±7 035、7d组为5 3107±15 357、10d组为60 095±17 388,均高于假手术组的5 474±1 311,差异均有统计学意义（P＜0.05）.胶质纤维酸性蛋白表达的检测结果与前列腺炎症反应程度在时相上基本一致.结论 弗氏完全佐剂刺激大鼠前列腺可引起炎性反应,导致L6-S1脊髓背角星形胶质细胞活化及其标志物胶质纤维酸性蛋白表达增强,提示星形胶质细胞可能参与了前列腺炎性疼痛的形成过程.

Objective To analyze the activation of astrocytes in the corresponding spinal cord after chemical irritation of the rat prostate and investigate whether the astrocytes is involved in the inflammatory pain from prostatitis. Methods Twenty four healthy male SD rats were randomly divided into two groups： 6 as sham operated group and the other 18 as Non-bacterial prostatitis models induced by injecting CFA to prostate. The rats in the Non-bacterial prostatitis model group were again randomly divided into 3 subgroups （6 in each group） ： CFA injection 3d group, CFA injection 7d group, and CFA injection 10 d group. The shape of glandular cavity, the extent of hemangiectasis and inflammatory cell infiltration were stained by H＆E and observed by light microscope. The changes in the integrated optical density （IOD） , the value of GFAP in L6-S1 spinal cord astrocytes of rats were detected and analyzed by immumofluorescence method combined with IPP 6 image measurement software. Results Our results showed that the inflammatory re- action of prostate in CFA injection 3d group was slight （ vascular dilatation and congestion was mild, slight infiltration of neutrophils and lymphocytes in the perivascular and interstitial compartments was observed） , while it was more obvious in CFA injection 7 d group （vascular dilatation and congestion was more serious with vascular rupture, significant infiltration of neutrophils and lymphocytes in the perivascular and intersti- tial compartments was observed） , in addition, the inflammatory reaction of prostate in CFA injection 10 d group was basically consistent with the 7 d group; Compared with sham operated group （5 474±1 311 ） , the expression of GFAP was higher in the model group （ CFA injection 3 d group was 24 805±7 035, CFA injec- tion 7 d group was 53 107±15 357, CFA injection 10 d group was 60 095±17 388） , which had statistically significant. The expression results of GFAP were basically consistent with the extent of inflammatory reaction of prostate in the time phase, Conclusions Inflammatory pain of the prostate caused by CFA lead to acti- vation of astrocytes in the dorsal horn of the corresponding （ L6 and S1 ） segments of the spinal cord and en- hanced expression of GFAP. These results suggest that the activation of astroeytes may be involved in the for- mation of the inflammatory pain of prostate.