miR-141在儿童急性B淋巴细胞白血病中的表达及意义

Expression and clinical significance of miR-141 in childhood B-cell acute lymphoblastic leukemia

背景与目的：微小RNA（microRNA，miRNA）是一类长度为21-25nt的内源性单链非编码RNAdx分子。本研究旨在探讨miR-141在儿童急性B淋巴细胞白血病（acutelymphoblasticleukemia，ALL）中的表达及临床意义。方法：收集B细胞ALL患）L35例为B细胞ALL组，以非血液病患）L15例为对照组，采集骨髓标本，提取总RNA，采用real-timePCR检测miR-141的表达，比较各组miR-141表达差异。结果：miR-141在B细胞ALL组中的表达低于对照组（P〈0．05）；miR-141在治疗前的表达明显低于治疗第30天及第12周（P〈0．05），治疗第30天的表达低于治疗第12周（P〈0．05）：≥i0岁组miR-141的表达低于〈10岁组（P〈0．05）；低危组治疗前miR-141表达高于中、高危组（P〈0．05），而中危组高于高危组护〈0．05）。结论：miR-141在儿童B细胞ALL中很可能存在抑癌作用，并是预后预测的潜在靶点。

Background and purpose： MicroRNAs are 19 to 25-nucleotide noncoding RNA molecules. The aim of this article was to investigate the expression and clinical significance of microRNA-141 in childhood B-cell acute lymphoblastic leukemia （ALL）. Methods： Bone marrow samples were collected from 35 children with B-cell ALL and 15 children with non hematologic disease. Total RNA was acquired from bone marrow. Real-time PCR was performed to detect the expression level of miR- 141. Results： The relative expression level of miR- 141 in B-cell ALL group was remarkably lower than those in the control （P〈0.05）. The expression ofmiR-141 in newly diagnosed samples was lower than those in Day 30 and Week 12 samples respectively （P〈0.05）. Besides, the expression ofmiR-141 in Day 30 samples was lower than those in week 12 samples （P〈0.05）. The expression of miR-141 in children over 10 years old was significantly lower than those in children under 10 years old （P〈0.05）. The expression of miR-141 in low-risk group was higher than those in mid-risk and high-risk group respectively （P〈0.05）, and there was significant difference between mid-risk and high-risk groups （P〈0.05）. Conclusion： MiR-141 is likely to have tumor suppressor effect and to be a ootential prognostic biomarker in childhood B cell ALL.