正畸牙移入对牙槽突裂植骨区骨改建的生物学影响

The biological effect of orthodontic tooth moving into alveolar cleft bone grafting area

目的 :通过检测正畸牙移入后牙槽突裂植骨区内Trap、Rankl、Runx2的表达,研究正畸牙移入对牙槽突裂植骨区骨改建的生物学影响。方法:选用12只8周龄SD雄性大鼠,制备正畸牙移入牙槽突裂植骨区的动物模型,加力前及加力1、3、5 d后处死动物取材,进行H-E染色,检测裂隙区骨形成情况,采用实时荧光定量PCR检测Trap、Rankl、Runx2表达,应用SPSS 13.0软件包对数据进行两样本均数t检验。结果:Trap在早、中期升高,Rankl在早期升高,Runx2在晚期升高。结论:正畸牙移入加强了植入骨的骨重建,诱导了破骨和以后的成骨过程。

PUPPOSE： To study the biological effects of orthodontic tooth movement into alveolar cleft bone graft area, and evaluate expression of Trap/Rank1/Runx2 by real-time PCR. METHOD： The model of orthodontic tooth movement into alveolar cleft bone graft area was established in 8-week-old SD rats. The animals were sacrificed before orthodontic treatment and 1, 3, 5 days after orthodontic treatment. Bone formation was evaluated by H-E staining and the expression of Trap/Rankl/Runx2 was detected by Real-time PCR. SPSS 13.0 software package was used for two sample paired t test. RESULTS： The expression of Trap increased at early and middle stage, Rankl increased at early stage and Runx2 increased at late stage. CONCLUSION： Orthodontic tooth movement into alveolar cleft bone graft area promoted bone remodeling of embedded bone, induced osteoclastic process and subsequent osteogenesis. Supported by Scientific Research Projects of Science and Technology Committee of Shanghai Municipality （124119b0103）.