p53上调凋亡调控因子在舌鳞癌中的表达及临床意义

PUMA expression and its clinical significance in tongue squamous cell carcinoma

目的:观察p53上调凋亡调控因子(p53 up-regulated modulator of apoptosis,PUMA)在舌鳞癌组织、舌癌旁组织及正常舌黏膜组织中的表达,探讨其在舌鳞癌发生、发展中的作用及临床意义。方法:通过免疫组化检测30例舌鳞癌组织、13例舌癌旁组织及10例正常舌黏膜组织中PUMA的表达水平,分析PUMA在舌鳞癌中的表达与临床分期、肿瘤分化程度、淋巴结转移之间的关系。采用SPSS17.0软件包对数据进行统计学分析。结果:PUMA在舌鳞癌组织、舌癌旁组织及正常舌黏膜组织中的阳性表达率分别为36.67%、53.85%和80.00%,3组之间PUMA表达均有显著差异(P<0.05)。淋巴结转移组中PUMA的阳性表达率为18.18%,而无淋巴结转移组中PUMA的阳性表达率为47.37%,2组差异显著(P<0.05)。而在不同年龄、性别、临床与病理分期、肿瘤分化程度的舌鳞癌组织中,PUMA的表达差异无显著性(P>0.05)。结论:PUMA蛋白表达降低在舌鳞癌的发生、发展及转移过程中具有重要作用,对判断舌鳞癌的预后及治疗有一定的指导意义。

PURPOSE： To investigate the expression of PUMA （p53 up-regulated modulator of apoptosis） in tongue squamous cell carcinoma （TSCC）, tongue tissues adjacent to cancer and normal tongue tissues, and to explore its clinical significance. METHODS： The expression of PUMA in resected samples from TSCC, tongue tissues adjacent to cancer and normal tongue tissues were detected by immunohistochemistry. The relationships between the expression of PUMA and the TNM staging, cell differentiation, and lymph node metastasis were analysed respectively. SPSS 17.0 software package was used for statistical analysis. RESULTS： Immunohistochemical studies demonstrated that PUMA expression was lower in TSCC （36.67%） than in tongue tissues adjacent to cancer （53.85%） and normal tongue tissues （80.00%）. There was significant difference of PUMA expression in lymph node metastasis and lymph node without metastasis （P〈0.05）. The expression of PUMA in TSCC had no relation with age, gender, clinical stages, and the degree of cell differentiation （P〉 0.05）. CONCLUSION： The results indicated that PUMA expression in TSCC is correlated with the pathogenesis and development of TSCC and lymph node metastasis and maybe a new promising target for treatment of TSCC. Supported by National Natural Science Foundation of China （30973340 and 81272554）, Guangdong Science and Technology Project （2011B050400030 and 2012B031800387） and Natural Science Foundation of Guangdong Province （9151008901000187 and S2011020003247）.