病理性瘢痕中整合素链激酶、磷脂酰肌醇3激酶蛋白和mRNA表达及其临床意义

Expression of integrin-linked kinase and phosphatidylinositols 3-kinase in pathological scar and their clinical significance

目的探讨正常皮肤、非病理性瘢痕及病理性瘢痕中整合素链激酶和磷脂酰肌醇3激酶的表达情况。方法统计整形外科病理性瘢痕40例，非病理性瘢痕12例，正常皮肤组织16例；采用免疫组织化学法和蛋白印迹及荧光实时定量PCR，检测3种组织中整合素链激酶和磷脂酰肌醇3激酶蛋白及mRNA表达情况。结果整合素链激酶蛋白在正常皮肤、非病理性瘢痕及病理性瘢痕中的阳性率分别是25．00％，41．67％，85．00％。磷脂酰肌醇3激酶蛋白在3种组织中的阳性率分别是12．50％，16．67％，80．00％；蛋白印迹及荧光实时定量PCR结果显示，与正常皮肤及非病理性瘢痕相比，病理性瘢痕中整合素链激酶、磷脂酰肌醇3激酶蛋白及mRNA表达均有统计学意义。病理性瘢痕中整合素链激酶与磷脂酰肌醇3激酶蛋白表达呈正相关（P=0．001，r=0．614）。结论整合素链激酶和磷脂酰肌醇3激酶蛋白可能通过磷脂酰肌醇3激酶／PKB信号通路参与病理性瘢痕的形成；整合素链激酶和磷脂酰肌醇3激酶蛋白协同促进病理性瘢痕的形成；整合素链激酶和磷脂酰肌醇3激酶蛋白靶向治疗阻止组织病理性瘢痕的形成有望成为可能。

Objective To explore the expression of integrin-linked kinase （ILK） and phosphatidylinosi- tols 3-kinase （PI3K） in normal skin tissue, mature scar and pathological scar and their clinical significance. Methods The expression protein and mRNA of ILK and PI3K were detected by immunological histological chemistry （IHC）, Western blot and fluorescent quatititive PCR, respectively. Results The positive rates of ILK proteins were 25.00%0 , 41.67%0 , 85.00% in normal skin tissue, mature scar and pathological scar, re- spectively. The positive rates of PI3K proteins were 12.50% , 16.67% , 80.00% , respectively. The expres- sion of protein and mRNA of ILK and PI3K were higher in pathological scar when compared to both normal skin tissue and mature scar, the data showed statistically significant. There was a highly positive correlation between ILK and PI3K protein expression （ P=0.001, R=0. 614）. Conclusion ILK, PI3K protein might be involved in the formation of pathologic scar through the activation of PI3K/PKB signaling pathway. PI3K and ILK pro- teins may cooperate to promote the formation and progress of pathologic scar. ILK and/or PI3K target treatment might be possible to prevent the formation of pathologic scar.