绿豆立枯丝核菌研究初报

Preliminary identification of Rhizoctonia solani causing mung bean seedling blight

本研究通过形态学、菌丝融合群和致病力测定研究,对从河北省石家庄地区绿豆种植区分离的90个立枯丝核菌进行鉴定。在90个分离物中有71个属于AG4,占供试分离物的78.89%,2个属于AG2-2,占供试分离物的2.22%,另外17个分离物与标准菌株不融合,占供试分离物的18.89%;属于AG4的71个分离物中,55个与AG4完全融合(占77.46%),16个与AG4不完全融合(占22.54%)。在温室条件下采用人工接菌法对40个代表性分离物的致病力进行测定,发现不同分离物对同一品种的致病力存在差异,其中分离物R3、R6、R9、R35致病力最强,分离物R23、R31-1致病力最弱。属于AG-4的分离物R3、R6、R9、R35与其他供试分离物致病力差异极显著;属于未知群体的分离物R20、R29和R24之间致病力差异极显著;属于AG2-2的分离物R21、R31-1致病力较弱,且差异不显著。

Ninety isolates of Rhizoctonia solani,collected from the mung bean field in Shijiazhuang region were characterized by morphology,anastomosis group and pathogenicity test.The results showed that all isolates were multinucleate.Seventy-one isolates belonged to the AG4,accounting for 78.89％ of the tested isolates,55 isolates were completely fused with AG4 （77.46％）,16 strains were incompletely fused with AG4 （22.54％）.Two isolates belonged to the AG2-2,accounting for 2.22％ There were 17 strains that could not fuse with any standard strains,accounting for 18.89％.Virulence test in greenhouse demonstrated that the isolates R3,R6,R9 and R35 were highly virulent,but isolates R23 and R31-1 were weakly virulent.There were extremely significant difference between the isolates R3,R6,R9,R35 and the others belonging to AG-4,also between the isolates R20,R29 and R24 belonging to unknown group.The isolates R21 and R31-1 belonging to AG2-2 were weakly virulent and had no significant difference in virulence.