亚致死剂量氯虫苯甲酰胺对小菜蛾体内活性酶的影响

Effects of sublethal concentrations of chlorantraniliprole on detoxifying enzyme activity in Plutella xylostella(L.)

为探讨亚致死剂量氯虫苯甲酰胺对小菜蛾[Plutella xylostella(L.)]体内活性酶的影响,采用叶片药膜法,用氯虫苯甲酰胺LC50和LC25剂量处理小菜蛾3龄幼虫24、48h后,测定了幼虫体内羧酸酯酶、多功能氧化酶、谷胱甘肽S-转移酶、乙酰胆碱酯酶等4种酶的活性变化。结果表明,亚致死浓度氯虫苯甲酰胺处理除对羧酸酯酶活性影响不显著外,对另3种酶活性均存在一定的抑制作用。谷胱甘肽S-转移酶活性下降最明显,LC25和LC50处理小菜蛾24h后,与对照相比分别下降92.57%和98.01%。

To clarify the effects of chlorantraniliprole at sublethal concentrations on the active enzymes of Plutella xylostella （L.）,the activities of 4 enzymes,carboxylesterase,function oxidase,glutathione S-transferase and acetylcholinesterase in P.xylostella were tested after the 3rd instar larvae were treated with sublethal doses of chlorantraniliprole （LC25 and LC50） by the leaf membrane bioassay.The results showed that 3 enzyme activities descended significantly except CarE,and glutathione S-transferase activity decreased the most obviously.When P.xylostella were treated with chlorantraniliprole at LC25 and LC50 for 24 h,the activity of glutathione S-transferase decreased by 92.57％ and 98.01％,respectively,compared with the control.