摘要
阵列毛细管电泳芯片检测系统的性能决定其并行检测的精度及可靠性。搭建出一种以CCD(TCD1304AP)为光电转换器件的阵列毛细管电泳芯片荧光检测系统,系统以LED为激发光源,可以同时检测4个通道内样品经分离后受光源激发所产生的荧光信号。系统光路部分利用LED光源、滤光片及针孔的有效组合,简化了光路结构;CCD器件将荧光信号转换为模拟信号,通过高速A/D转换后经USB接口发送至上位机进行实时显示。系统对不同浓度的Rhodamine B样品溶液进行了电泳分离实验,结果表明系统具有结构简单、实时性强、灵敏度较高等优点。
The accuracy and feasibility of parallel detection for capillary array electrophoresis microchip is determined by the performance of fluorescence detection system.A fluorescence detection system was presented in which CCD （TCD1304AP） and LED were used as photoelectric device and light source,respectively.The analog signals were transformed to digital signals by highspeed A/D device and then were sent to host computer for real-time display.Different concentrations of Rhodamine B sample solution were introduced into the microchannels for electrophoretic separation and the results showed that the system had advantages of compact structure,real-time process,and higher sensitivity
出处
《仪表技术与传感器》
CSCD
北大核心
2014年第3期61-64,共4页
Instrument Technique and Sensor
基金
河南省教育厅科学技术研究重点项目(13B510240)
关键词
TCD1304AP
毛细管电泳芯片
荧光检测
LED光源
电泳分离
TCD1304AP
capillary electrophoresis microchip
fluorescence detection
LED light source
electrophoretic separation
