RVVC缓解期阴道局部IL-4、IL-12、IFN-γ水平异常及临床意义初探

Study on level of IL-4,IL-12 and IFN-γ cytokines in vaginal lavage fluid of women with recurrent vulvovaginal candidiasis during paracmasia

目的:探讨复发性外阴阴道假丝酵母菌病(RVVC)缓解期女性阴道局部IL-4、IL-12、IFN-γ的水平。方法:采集缓解期RVVC女性卵泡期(RVVC缓卵泡期组)、黄体期(RVVC缓黄体期组)阴道灌洗液,以黄体期健康女性为正常对照(正常组),采用酶联免疫法(ELISA法)检测阴道局部IL-4、IL-12、IFN-γ水平。结果:IL-12和IFN-γ的水平在缓解期RVVC黄体期组均高于正常组(P<0.05),缓解期RVVC卵泡期组和正常组间相比无明显差异(P>0.05),缓解期RVVC卵泡期组和黄体期组间相比无明显差异(P>0.05);IL-4和IFN-γ/IL-4比值在三组间比较无差异(P>0.05)。结论:RVVC患者存在一定的阴道局部免疫功能失调,以黄体期表现明显,故推测在此期进行巩固治疗可以提高临床疗效。

Objective：To investigate the level of vaginal concentration of cytokines and vaginal local immunity in Recurrent Vulvovaginal Candidiasis （ RVVC ） during paracmasia. Methods： Vaginal lavage fluid （VLF） samples were obtained from 46 women with RVVC during paracmasia, of which 23 cases were during the follicular phase （ Follicular phase group） and 23 cases were during the luteal phase（luteal phase group）. The healthy women during the luteal phase act as the normal control. Concentration of IL-4, IL-12 and IFN-γ were measured using enzyme-linked immunosorbent assay kits. Data was analysed by One-Way ANOVA or Kruskal-Wallis tests ,P 〈 0.05 was considered significant. Results： Concentrations of IL-12 and IFN-γ in the VLF of women with RVVC during the lu- teal phase were significantly higher than the women of the normal control during paracmasia（ P 〈 0.05 ）. There was no difference be- tween the follicular phase of women with RVVC in remission and the control group in the concentration of IL-12 and IFN-γ The results did not differ between the women during the follicular phase and one during the luteal phase. The level of IL-4 and IFN-γ/IL4 among the three groups were not statistically different （ P 〉 O. 05 ）. Conclusion： These results indicate that the vagina local immunity function of RVVC female probably were disorder, especially during the luteal phase of the menstrual cycle, so we may had better choose this stage to consolidation therapy.