产多不饱和脂肪酸真菌的菌株筛选与鉴定

Screening and Identifying of Fungal Strains Producing Polyunsaturated Fatty Acids

经菌株分离培养,采用苏丹黑B染色法初筛、摇瓶法复筛的方法,从土壤中筛选得到16株具有较好合成油脂能力的真菌菌株。对16株菌株的产油量和脂肪酸组成分析表明,该16株菌株油脂组成中C18∶0、C18∶1和C18∶2含量都较高,其中菌株1.12、1.11、2.2、12.8、水3.33可以合成C20以上的脂肪酸。对产油脂菌株进行形态观察鉴定,确定为青霉属(Penicillium Link)、曲霉属(Aspergillus Micheliex Fr.)、拟青霉属(Paecilomyces Bainier)、梨孢帚霉属(Scopulariopsis Bainier)和枝孢属(Cladosprium Link)。

The sixteen fungi strains having a good ability of producing oils were screened form soil samples by the methods of isolated culture, preliminary screening with Sudan Black B stain and further screening by shake- flask culture. The oil output and fatty acid composition analysis showed that the sixteen trains had strong ability of synthesizing C18 fatty acids. Strains of 1.12, 1.11, 2.2, 12.8 and Shui3.33 can produce the C20 above of fatty acids. The strains of producing oils were identified as Penicillium Link, Aspergillus Mieheliex Fr. , Paecilomyces Bainier, Scopulariopsis Bainier, Cladosprium Link.