艾灸关元穴对大鼠力竭时间、肌乳酸及超氧化物歧化酶的影响

Effect of moxibustion at Guanyuan(CV-4) on exhaustive time, lactic acid and SOD in skeletal muscle of rats

目的:探讨艾灸关元穴对运动耐力的影响及其与自由基代谢的关系。方法:将大鼠随机分为正常对照组、力竭对照组、灸关元力竭组、运动训练力竭组,其中灸关元力竭组和运动训练力竭组分别接受艾灸(温和灸)和运动训练处理,20d后与力竭对照组一起测试游泳力竭时间;所有大鼠处死取材后用生化法检测并比较比目鱼肌乳酸和超氧化物歧化酶(SOD)含量。结果:灸关元力竭组和运动训练力竭组的平均力竭时间较力竭对照组延长(P<0.05,P<0.01)。力竭对照组乳酸较正常对照组升高(P<0.01),SOD较正常对照组降低(P<0.01)。灸关元力竭组和运动训练力竭组的乳酸较力竭对照组降低(P<0.01);SOD较力竭对照组升高(P<0.05,P<0.01)。结论:艾灸关元穴与运动训练一样,都能提高大鼠的运动耐力,减少肌乳酸堆积,其机制与骨骼肌SOD的含量变化有关。

Objective： To observe the effect of moxibustion at Guanyuan （CV-4） on exercise tolerance and its relation with free radical metabolism. Methods： Randomly divided rats into normal control group, exhausted control group, moxibustion group and swimming training group. After 20 days of intervention, rats in moxibustion group, swimming training group and exhausted control group received exhaustive swimming tests. Exhaustive time of each swimming rat was recorded. Lactic acid and SOD level in soleus muscle of each rat was detected. Results： The swimming exhausted time of both moxibustion group and swimming training group were significantly increased compared to exhausted control group （P〈0.05, P〈0.01）. The lactic acid of exhausted control group was significantly increased compared to normal control group （P〈0.01）, and the lactic acid of both moxibustion group and swimming training group were significantly lower than that of the exhausted control group （P〈0.01）. The SOD level of exhausted control group was significantly decreased compared to normal control group （P〈0.01）, and the SOD levels of both moxibustion group and swimming training group were significantly higher than that of the exhausted control group （P〈0.05, P〈0.01）. Conclusion： Like swimming training, moxibustion at Guanyuan （CV-4） can improve the exercise tolerance of rats and decrease the lactic acid accumulation. The physiological mechanism might be related to the SOD level in skeletal muscle.