BZL方对高脂饮食诱导大鼠非酒精性脂肪肝的防治作用及其对肝脏脂肪合成环节的影响

Preventive and therapeutical effect of BZL Decoction on nonalcoholic fatty liver disease of rats induced by high fat diet and infl uence on liver lipid synthesis

目的:在证实BZL方(白术多糖、栀子苷、绿原酸)对高脂饮食诱导的大鼠非酒精性脂肪肝(NAFLD)防治效应基础上,从肝脏脂肪合成环节探讨其作用机制。方法:采用高脂饮食诱导大鼠NAFLD动物模型。设正常组、模型组、BZL方组、祛湿化瘀方组、罗格列酮组,分别灌胃给药或饮用水4周。取材后观察和检测:体质量、肝湿重、肝指数等变化;血清丙氨酸转氨酶(ALT)、谷草转氨酶(AST)活性、肝组织γ-谷氨酰转移酶(γ-GT)活性变化;肝组织甘油三酯(TG)、游离脂肪酸(FFA)含量变化;运用Western blot,观察P-ACC、ChREBP和SCD-1蛋白表达水平。结果:与模型组比较,BZL方组ALT、AST、γ-GT活性以及肝组织TG、FFA含量显著性降低(P<0.01)。BZL方组P-ACC蛋白表达增强,同时ChREBP和SCD-1蛋白的表达都降低。结论:BZL方对高脂饮食诱导的大鼠NAFLD有显著的药效学效应,其药效优于祛湿化瘀方和罗格列酮。上调P-ACC蛋白的表达,同时降低ChREBP和SCD-1蛋白的表达,从而抑制肝脏脂肪合成可能是BZL方防治NAFLD的重要作用机制之一。

Objective： To study the effect of BZL Decoction （Atractylodes polysaccharide, Gardenoside and Chlorogenic acid） on nonalcoholic fatty liver disease （NAFLD） of rats induced by high fat diet and explore the mechanism of actions about liver lipid synthesis. Methods： Rats model of NAFLD, induced by high fat diet, were applied. Rats were randomly divided into normal group, model group, BZL Decoction group, Qushi Huayufang group and Rosiglitazone group. Five groups were respectively gavaged by medicines or drinking water for 4 weeks. Observation and test： changes of body weight, liver weight and liver index; ALT, AST, γ-GT, TG and FFA; the expression levels of P-ACC, ChREBP and SCD-1 were observed by Western blot. Results： Compared with model group, BZL Decoction could significantly decrease the activities of ALT, AST, y-GT and the contents of TG and FFA in liver tissue （P〈0.01）. P-ACC protein expression level in BZL Decoction group was higher, and protein expression levels of CHREBP and SCD-1 of BZL Decoction group were both lower. Conclusion： BZL Decoction has significant pharmacodynamics effects on nonalcoholic fatty liver of rats induced by high fat diet, and its efficacy is better than Qushi Huayufang＇ and Rosiglitazone＇. The function of up-regulating protein expression level of P-ACC, down-regulating protein expression level of ChREBP and SCD-1 and inhibiting liver fat synthesis might be one of the most important action mechanisms of BZL Decoction,