两种不同方法在新生儿溶血病诊断中的应用分析

Analysis of Two Different Methods in the Diagnosis of Hemolytic Disease of the Newborn

目的:观察比较酸放散与冷冻复融放散两种不同试验方法在新生儿ABO溶血病检测中的应用效果。方法:收集209例新生儿高胆红素血症血标本,同时进行酸放散和冷冻复融放散试验,比较两者间的差异及与临床诊断的吻合度。结果:酸放散阳性64例,阴性145例;冷冻复融阳性63例,阴性146例。两种检测方法总阳性率比较,差异无统计学意义(P>0.05),但在阳性程度上酸放散明显高于热放散。结论:酸放散是一种诊断新生儿ABO溶血病较好的检测方法。

Objective：To investigate the efficacy of acid elution and frozen-thawed elution test in detecting hemolytic disease of the newborn caused by ABO incompatibility.Method：209 cases of blood samples from newborns with hyperbilirubinemia were collected.Antibody molecules bound to red blood cell membrane were released by acid elution test or frozen-thawed elution test respectively.Then the difference and consistency with clinical diagnosis between two methods were analyzed.Result：In the group of using acid elution method,64 cases were positive（positive rate,30.62%）,and 63 cases were positive in frozen-thawed elution group（positive rate,30.14%）.There was no significant difference between two methods（P〉0.05）.However,the degree of positivity with acid elution was stronger than with frozen-thawed elution.Conclusion：Acid elution is a better method in diagnosing hemolytic disease of the newborn caused by ABO incompatibility.