黄连中盐酸小檗碱的提取工艺教学实验改进

Extraction of berberine from Rhizoma coptidis by inner ebullition

采用解吸-内部沸腾两步法改进大学专业实验黄连中盐酸小檗碱的提取工艺,得到最佳提取工艺如下:解吸剂浓度为40%乙醇、解吸剂固液比1:2、热提第一次时间1 min、第二次时间5 min、热提剂固液比1:40。并与传统醇提法相比,提取时间短、乙醇消耗少和浸膏有效成分含量高,有效的提高了实验效率和经济性。

The berberine was extracted by means of inner ebullition from rhizoma coptidis. The experimental results show that, the optimum technological conditions is：concentration of resolution is 40%ethanol, solid-to-liquid ratio of resolution is 1：2, the time of first extraction is 1 min, the time of second extraction is 5 min. Compared with the traditional ethanol extraction method, the inner ebullition extraction had the following obvious advantages that extraction time, ethanol consumption and yield of berberine. The inner ebullition extraction was rapid, effective and economic extraction method in the extraction of berberine from rhizoma coptidis.