亳州栽培知母最佳采收期研究

Study on Best Harvesting Time for Rhizoma Anemarrhenae Cultivated in Bozhou

目的 考察亳州栽培知母的最佳采收时间.方法 采用高效液相色谱-紫外光谱法和高效液相色谱-蒸发光散射检测器测定不同采收期知母中芒果苷和知母皂苷BⅡ的含量.测定芒果苷的流动相为乙腈-0.1％磷酸水溶液（15：85）,流速为0.8 ml/min,柱温25 ℃,检测波长为317 nm;测定知母皂苷BⅡ的流动相为乙腈-水（25∶75）,流速为1.0 ml/rnin,柱温25℃;蒸发光散射检测器的漂移管温度为60℃,载气压力30N/cm2.结果 不同采收期知母药材中芒果苷含量为1.10％～5.28％,知母皂苷BⅡ含量为0.22％～8.68％.结论 11月份是亳州栽培知母的最佳采收期,其芒果苷和知母皂苷含量最高.

Objective To determine the best harvesting time for Rhizoma Anemarrhenae cultivated in Bozhou, China. Methods High-performance liquid chromatography （HPLC） ultraviolet spectrometry and HPLC evaporative light-scattering detector （ELSD） were used to determine the content of mangiferin and timosaponin B-II in Rhizoma Anernarrhenae harvested at different times. For the determination of mangiferin, the mobile phase was acetonitrile-0.1% phosphoric acid （15：85）, with a flow rate of 0.8 ml/min; the column temperature was 25℃ ; the detection wavelength was 317 nm. For the determination of timosaponin B-II , the mobile phase was acetonitrile-water （25： 75）, with a flow rate of 1.0 ml/min; the column temperature was 25℃ ; the drift tube temperature of ELSD was 60℃ ; the carrier gas pressure was 3.0 N/cm2. Results The content of mangiferin and timosaponin B- II in Rhizoma Anemarrhenae harvested at different times was 1.10% -5.28% and 0.22% -8. 68% , respectively. Conclusion The best harvesting time for Rhi- zoma Anemarrhenae cultivated in gozhou is in：~ November, ：when it has ＇the highest content of mangiferin and timosaponin.