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一个杨树GDSL基因组织表达的特性及其在拟南芥异源的表达 被引量：2

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摘要 GDSL酯酶为脂肪水解酶家族的一个分支,参与植物生长发育和防御反应等多种功能。半定量RT-PCR分析结果显示,毛果杨GDSL酯酶基因Potri.002G253400在顶端茎组织中高丰度特异性表达;构建Potri.002G253400-GFP融合的植物表达载体,转化模式植物拟南芥并获得其过量表达的转基因株系7个;激光共聚焦显微镜检测结果显示,GFP荧光蛋白高丰度表达于转基因植株根的细胞壁区域,说明Potri.002G253400蛋白可能定位于细胞壁。
作者 奈婕菲 程玉祥
机构地区 东北林业大学/林木遗传育种国家重点实验室
出处 《江苏农业科学》 北大核心 2014年第3期16-18,共3页 Jiangsu Agricultural Sciences
基金 国家高技术研究发展计划(编号:2013AA102702)
关键词 毛果杨 拟南芥 GDSL酯酶 载体构建 转基因
分类号 S792.110.4 [农业科学—林木遗传育种]
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参考文献16

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二级参考文献3

  • 1WEIGEL D,GLAZEBROOK J.Arabidopsis:a laboratory manual[M].New York:Cold Spring Harbor Laboratory Press,Cold Spring Harbor,2002.
  • 2YAMADA K,LIM J,DALE JM,et al.Empirical analysis of transcriptional activity in the Arabidopsis genome[J].Science,2003,302:842-846.
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江苏农业科学
2014年 第3期

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