摘要
综述实时荧光定量PCR(qPCR)技术在重组腺相关病毒(rAAV)的基因组滴度测定中的应用成果,以及qPCR技术在rAAV质量控制过程中的应用,如感染滴度及rcAAV污染率的测定等.分析qPCR技术存在的适用范围窄、易产生系统性误差和易受rAAV杂质干扰等问题的原因,并探讨相关的解决策略.
This paper reviews the application achievement of real-time quantitative PCR (qPCR) technology in the re- combinant adeno-associated virus genome titer determination and its application in rAAVs' quality control, including in- fectious titer and contamination rate of reAAV. The current several problems of qPCR technique, such as relatively nar- row application range, easy to cause systemic errors and be interfered by rAAV impurity, are analyzed and discussed on better countermeasures.
出处
《华侨大学学报(自然科学版)》
CAS
北大核心
2014年第2期191-195,共5页
Journal of Huaqiao University(Natural Science)
基金
国家自然科学基金资助项目(81371669
81271691)
国家国际科技合作项目(2011DFG33320)