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重组腺相关病毒质量控制的qPCR技术研究进展 被引量：4

Advances in Real-Time Quantitative PCR Technology for Quality Control of Recombinant Adeno-Associated Virus
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摘要 综述实时荧光定量PCR(qPCR)技术在重组腺相关病毒(rAAV)的基因组滴度测定中的应用成果,以及qPCR技术在rAAV质量控制过程中的应用,如感染滴度及rcAAV污染率的测定等.分析qPCR技术存在的适用范围窄、易产生系统性误差和易受rAAV杂质干扰等问题的原因,并探讨相关的解决策略. This paper reviews the application achievement of real-time quantitative PCR （qPCR） technology in the re- combinant adeno-associated virus genome titer determination and its application in rAAVs＇ quality control, including in- fectious titer and contamination rate of reAAV. The current several problems of qPCR technique, such as relatively nar- row application range, easy to cause systemic errors and be interfered by rAAV impurity, are analyzed and discussed on better countermeasures.
作者 肖桂清 杨会勇 刁勇
机构地区 华侨大学分子药物研究院
出处 《华侨大学学报（自然科学版）》 CAS 北大核心 2014年第2期191-195,共5页 Journal of Huaqiao University(Natural Science)
基金 国家自然科学基金资助项目(81371669 81271691) 国家国际科技合作项目(2011DFG33320)
关键词 重组腺相关病毒 基因组滴度 实时荧光定量PCR 基因治疗 recombinant adeno-associated virus genome titratiom real-time quantitative PCR gene therapy
分类号 Q81 [生物学—生物工程]
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参考文献34

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二级参考文献33

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共引文献9

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引证文献4

二级引证文献5

华侨大学学报（自然科学版）
2014年 第2期

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