餐厨垃圾发酵产油脂的复合酶制剂水解试验

Test conditions of enzymatic hydrolysis of kitchen garbage for microbial oils production

为使餐厨垃圾能有效地通过微生物发酵产油脂,采用复合酶对餐厨垃圾进行水解预处理研究。研究结果表明,复合酶中淀粉酶、糖化酶、蛋白酶和纤维素酶投加量分别为92.5,1 250,3 000,100活力/g原料时,效果最优;其水解产物中氨基酸态氮浓度为137.7mg/L,还原糖的质量百分比含量为11.11%。在复合酶各组分投加量一定时,水解效果受pH值、温度和水解时间影响。试验结果表明,pH值为6、温度为55℃、水解时间为30min时,水解效率最高;在最佳水解条件下,每升水解液经从土壤筛选分离的菌种B发酵7d后,生物产量36.9g,微生物油脂产量5.172g。

The aim of present paper was to hydrolyze the useful ingredients of food waste by complex enzyme,which was used in the fermentation by strain B isolated from soil for lipid accumulation.The results show that：optimal complex enzyme dosage was 92.5μ/g raw of amylase,1 250μ/g raw of glucoamylase,3 000μ/g raw of protease and 100μ/g raw of cellu-lose,with the amino nitrogen production to 137.7 mg/L and reducing sugar yield of 11.11%.While in a certain dosage of complex enzyme,pH value,temperature and hydroly-sis time could affect the hydrolysis.Single factor experiment showed：the condition of most efficient hydrolysis was pH value of 6,temperature was 55 ℃ and hydrolysis time was 30 min.Hydrolyzate which obtained in the optimal hydrolysis conditions fermentation by strain B for 7 days,the biomass of strain B and lipid yield could reach up to 36.9 g and 5.172 g per liter hydrolyzate.