人参二醇组皂苷对内毒素休克大鼠肺损伤的保护作用

Protective effect of Ginsen panaxadiol saponins in rat with endotoxic shock-induced lung injury

目的观察人参二醇组皂苷(ginsen panaxadiol saponins,PDS)对内毒素休克大鼠肺组织结构及肺内血栓素B2(TXB2)及6-酮-前列腺素F1α(6-Keto-PGF1α)含量的影响,探讨其对内毒素休克性肺损伤的保护作用。方法实验动物随机分为对照组(Control组)、内毒素休克组(LPS组)、地塞米松(LPS+Dex组)组和人参二醇组皂苷组(LPS+PDS组)。通过舌下静脉注射PDS对大鼠进行预治疗,10 min后再给予细菌内毒素(LPS 5 mg/kg)复制内毒素休克模型。休克后4 h处死动物,取肺组织固定并制备切片,观察其组织结构的变化;另外取20 mg肺组织匀浆,进行6-Keto-PGF1α和TXB2含量的检测。结果 LPS组的肺组织可见较弥漫的间质炎性改变,LPS+PDS中剂量组的肺组织内未见明显间质炎性改变;肺组织中的6-Keto-PGF1α含量及6-Keto-PGF1α/TXB2在LPS+PDS中剂量组高于LPS组。结论 PDS可通过调节肺内TXB2及6-Keto-PGF1α的含量,协调二者的平衡关系,对内毒素休克大鼠的肺损伤起到改善和保护作用。

[ Objective] To investigate the protective effects of Ginsen panaxadiol saponins （PDS） on endo- toxic shock-induced lung injury. [Methods] Rats were given pre-treatments by sublingual venous injection of PDS and were injected lipopolysaccharide （LPS 5 mg/kg） 10 min later to generate the septic shock models. Experimental rats were randomly divided into LPS, LPS ＋ dexamethasone （Dex）, LPS ＋ PDS （22.5 mg/kg, 45 mg/ kg, 90 mg/kg） and control groups respectively. Pathological alteration of lung tissues was observed in the shocked rats of different groups. Animals were taken lung to be stained and measure PGF1 α and TXB2 4 h after shock. [Results] Diffuse inflammatory of pulmonary interstitium was observed in LPS group. But there is no significant change in LPS ＋ PDS group. The levels of 6-Keto-PGF1 α and 6-Keto-PGF1 α/TXB2 in- creased obviously with comparison of LPS group. [Conclusion] PDS adjust the content of TXB2 and 6-Keto- PGF1 α in the body of LPS-shock rats and protect lung injury caused by endotoxic shock.