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分子动力学和丙氨酸变异研究MDM2与抑制剂P4的结合模式 被引量：1

Insight into binding mode of inhibitor P4 to MDM2 based on molecular dynamics and alanine mutation calculations
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摘要 抑制p53-MDM2相互作用已经成为癌症治疗的新途径.采用分子动力学模拟和MM-PBSA(molecular mechanics-Possion-Boltzmann surface area)方法研究了肽类抑制剂P4与MDM2的结合模式.研究表明P4与MDMD2疏水性裂缝的范德华作用是抑制剂结合的主导力量。采用丙氨酸变异计算研究了P4与MDM2的作用热区.结果表明残基Lys51,Leu54,Leu57,Ile61,Met62,Tyr67,Gln72,His73,Val93,His96和Ile99的丙氨酸变异导致了范德华作用的降低,而对极性相互作用几乎没有产生影响,同时也证明这些残基处于P4与MDM2作用表面的热区,对抑制剂的结合有重要贡献,这能为抗癌药物的设计提供理论上的指导. Inhibition of p53-MDM2 interaction has been a new approach curing cancers.Molecular dynamics simulation coupled with molecular mechanics-Poisson-Boltzmann surface area （MM-PBAS） method was performed to study the binding mode of inhibitor P4 to MDM2.The results show that van der Waals interactions of P4 with the hydrophobic cleft of MDM2 drive inhibitor binding.Computational alanine scanning mutagensis was adopted to explore the hot spots of P4-MDM2 interaction.The results suggest that alanine mutations of residues Lys51,Leu54,Leu57,Ile61,Met62,Tyr67,Gln72,His73,Val93,His96 and Ile99 from MDM2 lead to a big decrease in van der Waals energy,but hardly produce effects on polar interactions.At the same time,this result also proves that these residues locate in the hot spots of interaction surface between P4 and MDM2,and provide important contributions to inhibitor binding.This study can provide significant helps for anti-cancer drug designs.
作者 陈建中 梁志强 王伟 时术华 张少龙 段莉莉 张庆刚
机构地区 山东交通学院理学院 山东建筑大学理学院 山东师范大学物理与电子科学学院
出处 《原子与分子物理学报》 CAS CSCD 北大核心 2014年第2期311-316,共6页 Journal of Atomic and Molecular Physics
基金 国家自然科学基金(11104164 11274206 31200545) 山东省自然科学基金(ZR2011HM048) 山东交通学院博士启动资金和校自然基金
关键词 分子动力学 丙氨酸变异 MM-PBSA p53-MDM2相互作用 Molecular dynamics Computational alanine scanning mutagensis MM-PBSA p53-MDM2 interaction
分类号 Q641.12 [生物学—生物物理学]
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参考文献20

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共引文献13

同被引文献20

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原子与分子物理学报
2014年 第2期

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