一种袋栽黑木耳共生菌的鉴定及其共生效应初步研究

Identification of a symbiotic fungus of bag-cultured Auricularia auricula and preliminary studies on its symbiotic effect

【目的】研究袋栽黑木耳某种共生菌对黑木耳生长的影响。【方法】经分离纯化,以及形态学和分子系统学分析,鉴定该真菌的分类地位;通过不同时间接种共生菌到黑木耳菌棒的方法,研究两者的共生效应;通过子实体营养成分分析,研究共生菌对黑木耳子实体营养结构的影响。【结果】经分离纯化获得菌株B-1,形态学和分子系统学分析将该菌株鉴定为毛色二孢属的可可毛色二孢(Lasiodiplodia theobromae)。通过不同时间接种黑木耳菌棒发现,此真菌与黑木耳间存在偏性共生关系。它对于黑木耳是偏利共生,还是偏害共生取决于黑木耳菌丝在菌棒中是否具有先占优势。黑木耳子实体营养成分分析显示,可可毛色二孢的存在改变了黑木耳子实体中营养结构,但黑木耳子实体形态特征无明显变化。【结论】可可毛色二孢与袋栽黑木耳存在着偏性共生关系,该现象为首次报道。

[Objective] Identification of a symbiotic fungus of bag-cultured Auricularia auricula and preliminary studies on its symbiotic effect. [Methods] After purified the symbiotic fungus strain, it was identified by morphology and molecular biological analysis. The symbiotic fungus was inoculated at different stages of bag-cultured A. auricula to explain the relationship between the two fungi. The nutritional analysis of fruit bodies ofA. auricula to explore the influence of the symbiotic fungus on nutritional structure of fruit bodies of A. auricula. [Results] The symbiotic fungus strain B-1 was identified as Lasiodiplodia theobromae. The inoculation experiment confirmed that the partial symbiosis between the A. auricula and strain B-1. The nutrition components of fruit bodies ofA. auricula analysis found that it changed significantly in the case of the presence of strain B-I, and no obvious changed in morphology. [Conclusion] A. auricula and L. theobromae strain B-1 have the relationship of partial symbiosis, which is first report.