三穗鸭肌肉生长抑制素基因的生物信息学分析

Bioinformatics Analysis of MSTN Gene in Sansui Duck

采用PCR产物直接测序的方法获得肌肉生长抑制素(myostatin,MSTN)基因的编码区序列,并运用生物信息学方法对该基因编码蛋白的理化性质、信号肽、跨膜结构、二级结构、三级结构和功能结构域进行分析。结果表明,三穗鸭MSTN蛋白是一个疏水性不稳定蛋白,作为信号肽的可能性很大;含有22个α螺旋、26个β折叠、26个T转角、20个无规则卷曲,有1个跨膜结构,具有TGF-β的主要结构。研究结果为MSTN基因的进一步研究提供参考。

In this study, the coding region of MSTN gene was amplified by PCR and sequenced by direct sequencing. Some characters of the MSTN gene encoding protein, including the physico chemical properties, singal peptide, ransmembrane structure, secondary structure, high structure and functional domains, were analyzed by bioinformatics tools. The results showed that MSTN protein of Sansui duck was a kind of labile protein with high hydrophobicity, which was probably being singnal peptide. The protein contained 22 a-helics,26 β-sheets,26 T-turns,20 irregular coils and 1 transmembrane domain, and it had the main feature of TGF-β superfamily. The result provided an important reference for its further research.