犬脂肪间充质干细胞的分离培养及分化潜能研究

Isolation,Culture and Differentiation Potential of Canine Adipose-derived Mesenchymal Stem Cells

为获得犬脂肪间充质干细胞,本试验取犬腹股沟皮下脂肪组织,分别利用组织培养法和酶消化法分离犬脂肪来源间充质干细胞,对比观察不同来源细胞的形态和增殖特征,并通过诱导液促进细胞向成骨细胞和成脂细胞方向分化,检测其分化潜能。结果表明,通过组织培养法培养的青年犬脂肪组织,可获得大量脂肪间充质干细胞,该细胞生长旺盛,形态均一,可分化为碱性磷酸酶染色阳性的成骨细胞和油红O染色阳性成脂细胞。组织培养法分离培养犬脂肪间充质干细胞操作简单,可为细胞移植治疗等研究提供充足的细胞来源。

In order to obtain canine adipose-derived mesenchymal stem cells （ASCs）, the dog inguinal subcutaneous adipose tissue was used to isolate ASCs by the methods of tissue culture and enzyme digestion, respectively. Different cell morphologies and proliferation characteristics were observed and the differentiation potential was tested by inducing cells into osteoblasts and adipocytes. The results showed that the adipose tissue from young dogs could get a lot of ASCs by tissue culture methods, which grew strong, uniform morphology, and could differentiate into osteoblasts with alkaline phosphatase positive and adipocytes with oil red O positive. The isolation and culture method of canine ASCs was easy to be established and could provide suf ficient cell sources for the study of cells transplantation and therapy.