摘要
研究选取中药单体18β-甘草次酸联合一线抗结核药物异烟肼（INH）、利福平（RFP）和链霉素（SM），利用MABA法检测其体外单独及联合作用于牛源结核分枝杆菌的抗菌活性。18β-甘草次酸单独作用于结核分枝杆菌标准株（ATCC27294）和牛分枝杆菌标准株（ATCC19210）的最低抑菌浓度（MIC）分别为50和100ug／mL。临床分离的2株敏感株和6株耐药株的MIC值分别为25-50和100～200ug／mL。18β-甘草次酸联合INH、RFP和SM作用于6株耐药株均具有协同作用，且MIC值明显下降，INH的MIC值下降2～32倍（FICIs 0．125～0．375），RFP的MIC值下降4～8倍（FICIs 0．240～0．490），SM的MIC值下降4～16倍（FICIs 0．165～0．460）。中药单体药物对正常细胞BHK-21具有较低的细胞毒性，对肝癌细胞SMMC具有较好抑制作用。结果表明，18p甘草次酸与抗结核药物INH、RFP和SM联合使用对结核分枝杆菌具有较好的抗菌药理活性。
In this study,we found that 18β-glycyrrhetinic acid had potent antimycobacterial properties against the drug-sus- ceptible and drug-resistant Mycobacterium boris. Minimum inhibitory concentration （MIC） were 50 and 100 ug/mL after appling 18β-glycyrrhetinic acid alone aginst ATCC 27294 and ATCC 19210; MICs were 25-50 and 100-200 ug/mL after appling 18β-glycyrrhetinic acid alone aginst 2 drug-susceptible and drug-resistant Mycobacteriumbovis. More importantly, they exhibi- ted synergistic effects with the first-line drugs isoniazid （INH）, rifampicin （RIF） andstreptomycin （SM） against clinical Myco- bacterium boris isolates, including drug-resistant strains. In combination with asubinhibitory concentration of 18β-glycyrrhetinic acid, the minimum inhibitory concentrations （MICs） of the antituberculosisagents decreased, ranging from 2 to 32, 4 to 8 and 4 to 16 fold for INH （fractional inhibitory concentrationindex （FICIs） 0. 125-0. 375）, RIF （FICIs 0. 240-0. 490） and SM （FICIs 0. 165-0. 460）, 18β-glycyrrhetinic acid had lesser poisonous to BHK-21 cell and could restraint SMMC fission,respectively. These findings indicated that 18β-glycyrrhetinic acid might serve as potential therapeutic compounds for futureantimycobacterial drug development.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第4期160-164,共5页
China Animal Husbandry & Veterinary Medicine
基金
内蒙古自治区高等学校科学研究项目(2013NJZY3383)
