摘要
从鲫鱼肠道中分离出1株细菌，暂时编号为SR-1，进行细菌形态学观察、理化特征、16SrDNA序列分析及药敏试验等研究，结果表明SR-1菌株为山梨醇发酵阳性的温和气单胞菌；PCR扩增SR-1菌株的16SrDNA序列为1509bp；BLAST比对分析结果表明SR-1菌株与温和气单胞菌（Aeromonassobria）亲缘关系最近，序列同源性为99．87％～100％；药敏试验结果显示SR～1菌株对阿奇霉素、氯霉素、四环素、诺氟沙星、头孢噻肟、头孢克肟等药物敏感。
A bacteria was isolated from the intestine of Carassius auratus and temporarily named as SR-1 strain with sorbitol-positive character. It was identified as Aeromonas sobria by morphological, physiological, biochemical characteristics, drug sennsitivity test, 16S rDNA sequence and phytogenetic tree analysis. Further the 16S rDNA sequence of SR-1 was amplified by PCR method and the results showed that the size of fragment was 1509 bp. Phylogenetic tree analysis showed that SR-1 strain had the highest similarity to Aeromonas sobria of 99.87% to 100% . Drug sensitivity test results revealed that SR-1 was sensi- tive to azithromycin, chloroamphenicol, tetracycline, norfloxacin, cefotaxime, cefixime and so on.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第4期241-244,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(31272692
30800847)
关键词
鲫鱼
温和气单胞菌
分离鉴定
药敏试验
系统进化树
Carassius auratus
Aeromonas sobria
isolation and identification
drug sensitivity test
phylogenetic tree
