救必应不同提取物与西药联合对产ESBLs细菌作用研究

Study on the Effect of Different Holly bark Extracts Combined with Western Medicine on ESBLs-producing Bacterial

试验旨在探讨4种救必应提取物与抗菌药物联合诱导细菌传代的体外抑菌活性。本试验制备了4种救必应提取物,采用96孔反应板二倍微量稀释法体外检测提取物与抗菌药物联合诱导耐药大肠杆菌传代的抑菌作用。结果显示,4种救必应提取物与抗菌药物联合诱导细菌传代对耐药大肠杆菌有不同程度的抑菌活性,其中提取物Ⅰ、Ⅲ与抗菌药物联合效果最好。提示,救必应提取物Ⅰ、Ⅲ与抗菌药物联合诱导细菌传代具有体外抗耐药菌作用。

This research was aimed to investigate four kinds of Holly bark extracts combined with antimicrobial drugs to induce bacteria in vitro antibacterial activity. Four kinds of Holly bark extracts were prepared, the bacterial passage of drug resistant Escherichia coli induced by Holly bark extracts combined with antimierobial drugs were tested by 96-well reaction plate twice micro-dilution method. Four kinds of Holly bark extracts combined with antimierobial drugs to induce bacteria passage for drug-resistant Escherichia coli had different antibacterial activities, in which extracts I , III combination with antimicrobial drugs had the best effect. Holly bark extracts I , III combined with antimicrobial drugs to induce bacterial passage bad antidrug-resistant bacteria effect in vitro.